Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,863,059 |
G→A |
L56L (CTG→CTA) |
ygbM → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,863,059 | 0 | G | A | 100.0%
| 164.2
/ NA
| 49 | L56L (CTG→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/33); total (16/33) |
CCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGC > W3110S.gb/2863000‑2863115
|
cccTATAACTACTCCCCCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:4187244/1‑62 (MQ=255)
cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:563871/1‑62 (MQ=255)
cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:3822097/1‑62 (MQ=255)
cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:389109/1‑62 (MQ=255)
ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:2653365/61‑1 (MQ=255)
ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:3814772/61‑1 (MQ=255)
ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:206852/61‑1 (MQ=255)
cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt < 1:2156656/49‑1 (MQ=255)
cccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt < 1:1923090/49‑1 (MQ=255)
ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCt < 1:3298220/48‑1 (MQ=255)
ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc < 1:4074004/62‑1 (MQ=255)
ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc < 1:1854332/62‑1 (MQ=255)
ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc < 1:496846/62‑1 (MQ=255)
ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTc < 1:1108515/62‑1 (MQ=255)
cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:90172/41‑1 (MQ=255)
cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:4203686/41‑1 (MQ=255)
cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc < 1:437060/41‑1 (MQ=255)
aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt > 1:1594540/1‑49 (MQ=255)
aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt > 1:280248/1‑49 (MQ=255)
aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAAt > 1:2213292/1‑49 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGgaga > 1:2717828/1‑61 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat > 1:730616/1‑62 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat > 1:663295/1‑62 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat > 1:65407/1‑62 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGat > 1:249539/1‑62 (MQ=255)
tCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGAGCTGTTCAATACCGCTCCCGgag > 1:3592594/1‑60 (MQ=255)
ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg < 1:3619619/57‑1 (MQ=255)
ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg < 1:1789694/57‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:2621501/62‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:1715176/62‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:2006752/62‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:3601358/62‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:3435843/62‑1 (MQ=255)
aGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:3365179/62‑1 (MQ=255)
gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:3639684/1‑62 (MQ=255)
gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:751154/1‑62 (MQ=255)
gCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:745793/1‑62 (MQ=255)
aCTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCgg < 1:76494/48‑1 (MQ=255)
aGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAAt < 1:893987/53‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGTAATg < 1:1729151/62‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:1006300/62‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:768517/62‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:2743349/62‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:3983318/62‑1 (MQ=255)
cAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:2848272/62‑1 (MQ=255)
aaaaCCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:3184101/61‑1 (MQ=255)
aaaCCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATg < 1:1323958/60‑1 (MQ=255)
acTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGc < 1:4549110/60‑1 (MQ=255)
acTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGc < 1:1922567/60‑1 (MQ=255)
|
CCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGC > W3110S.gb/2863000‑2863115
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
