Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,139,938 |
C→T |
R2H (CGT→CAT) |
hybD ← |
predicted maturation element for hydrogenase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,139,938 | 0 | C | T | 100.0%
| 175.7
/ NA
| 53 | R2H (CGT→CAT) | hybD | predicted maturation element for hydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/25); total (28/25) |
CGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAC > W3110S.gb/3139881‑3139995
|
cGCATACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:2929274/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:3674616/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:3784926/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:4022122/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:671916/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:1307808/61‑1 (MQ=255)
cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa < 1:577942/61‑1 (MQ=255)
cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACa < 1:4342164/62‑1 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGCCCCCTAAGACTAAAATATGCATTACAGa > 1:1113057/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAg > 1:1403655/1‑60 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:3172641/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:2824707/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:2483905/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:239083/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:1690677/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:1693159/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:1199790/1‑61 (MQ=255)
cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGa > 1:4215555/1‑61 (MQ=255)
tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt < 1:4052877/62‑1 (MQ=255)
tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt < 1:3004769/62‑1 (MQ=255)
tGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCt < 1:4514728/62‑1 (MQ=255)
ggCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:4669058/62‑1 (MQ=255)
gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:3748300/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:4219678/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:1011420/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:3454957/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:1537514/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:2991506/61‑1 (MQ=255)
ttCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCa < 1:1462523/61‑1 (MQ=255)
tCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCa < 1:1597969/62‑1 (MQ=255)
aaTATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:1868371/44‑1 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:895207/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:449542/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:947414/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:2386784/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:1956511/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:1602369/1‑62 (MQ=255)
atatTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTg > 1:1310850/1‑62 (MQ=255)
tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc < 1:2832558/53‑1 (MQ=255)
tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc < 1:2904665/53‑1 (MQ=255)
tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc < 1:1864490/53‑1 (MQ=255)
tAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc < 1:1682327/53‑1 (MQ=255)
aaaTATGCATTACAGAACCTTCACTGAACCCACTTCGTTGCCGTCAGCATCCACTACgtgt > 1:2452854/1‑61 (MQ=255)
aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt > 1:1327307/1‑61 (MQ=255)
aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:1133907/1‑62 (MQ=255)
aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:127999/1‑62 (MQ=255)
aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:1994603/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:18920/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:1201302/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:584994/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:2290508/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:246963/1‑62 (MQ=255)
aaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:3342631/1‑62 (MQ=255)
|
CGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAC > W3110S.gb/3139881‑3139995
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
