Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,294,438 |
T→C |
L395S (TTG→TCG) |
yraM → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,294,438 | 0 | T | C | 100.0%
| 258.9
/ NA
| 69 | L395S (TTG→TCG) | yraM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (42/27); total (42/27) |
CAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAA > W3110S.gb/3294383‑3294489
|
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:3231144/61‑1 (MQ=255)
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:1817242/61‑1 (MQ=255)
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:2469384/61‑1 (MQ=255)
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:2407264/61‑1 (MQ=255)
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:3813022/61‑1 (MQ=255)
cagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGa < 1:2097083/61‑1 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGTAGAGTCGCTGaa > 1:2704779/1‑59 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:2224341/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:2925433/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:2758054/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:3277038/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:2306996/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:3515011/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:3875462/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:1908172/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:4402612/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:17298/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:4565549/1‑61 (MQ=255)
gATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAaga > 1:798627/1‑61 (MQ=255)
aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:2259767/62‑1 (MQ=255)
aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:3459830/62‑1 (MQ=255)
aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:4469659/62‑1 (MQ=255)
aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:3093224/62‑1 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:940987/1‑62 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:3373071/1‑62 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:3241906/1‑62 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:109499/1‑62 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:2947528/1‑62 (MQ=255)
aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTcc > 1:2595860/1‑62 (MQ=255)
tcggtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTa > 1:3701116/1‑62 (MQ=255)
gtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACt > 1:3960333/1‑61 (MQ=255)
gtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACt > 1:1840665/1‑61 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgt < 1:1492270/62‑2 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:3313426/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:2141259/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:4039318/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:4041923/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:4366884/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:4392502/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:2676753/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:1541284/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:3076167/62‑1 (MQ=255)
tcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:521311/62‑1 (MQ=255)
ccGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:3474867/61‑1 (MQ=255)
cGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:3427540/60‑1 (MQ=255)
ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAAc > 1:4224669/1‑51 (MQ=255)
ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg > 1:1967630/1‑52 (MQ=255)
ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg > 1:129103/1‑52 (MQ=255)
ttGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg > 1:3023361/1‑52 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:3830356/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:4022396/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:2363713/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:2559984/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:3214875/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:1563485/1‑37 (MQ=255)
tGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCaa > 1:277474/1‑37 (MQ=255)
gCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGc < 1:398001/44‑1 (MQ=255)
gCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGc < 1:559377/44‑1 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:2964317/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:2977622/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:715953/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:710445/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:654198/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:451568/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:2616534/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:3373836/1‑61 (MQ=255)
aaTAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGc > 1:2027105/1‑61 (MQ=255)
gTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGaa < 1:3393117/62‑1 (MQ=255)
tGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:594372/41‑1 (MQ=255)
|
CAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAA > W3110S.gb/3294383‑3294489
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
