Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,459,280 |
T→C |
E15G (GAA→GGA) |
secE ← |
preprotein translocase membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,459,280 | 0 | T | C | 100.0%
| 244.2
/ NA
| 65 | E15G (GAA→GGA) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/19); total (46/19) |
GACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCA > W3110S.gb/3459231‑3459337
|
gACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCgcgc < 1:999353/61‑1 (MQ=255)
ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCGAGGCCGCGCCCGCTTCCTTGa < 1:841069/62‑1 (MQ=255)
ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa < 1:1165553/62‑1 (MQ=255)
ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa < 1:1660446/62‑1 (MQ=255)
aGGAGCAATGCCACCACAACGCCCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:3357248/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:3069643/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:4382686/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:2208866/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc < 1:2001333/62‑1 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:404787/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:1403585/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:613599/1‑62 (MQ=255)
aGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGc > 1:2966125/1‑62 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:367451/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:3974421/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:2592524/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:2046858/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:2109504/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:1611328/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:1394043/53‑1 (MQ=255)
ggAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:1009136/53‑1 (MQ=255)
gAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:4609862/52‑1 (MQ=255)
aGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:4165673/51‑1 (MQ=255)
cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGa < 1:1143046/46‑1 (MQ=255)
cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:622897/1‑61 (MQ=255)
cacaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:1955349/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2745349/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:459326/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:683151/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:4595957/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:3434987/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:3408353/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2764738/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:772296/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2629271/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2449942/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2249276/1‑61 (MQ=255)
caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:2177808/1‑61 (MQ=255)
caAAGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACt > 1:3909408/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:2391785/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:59529/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:1215967/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:1733287/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:1761575/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:2031860/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:4517589/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:4465054/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:699493/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:4093242/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:4065129/1‑61 (MQ=255)
aaCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTc > 1:3966802/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:789933/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:1071127/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:71763/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:536398/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:4378425/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:2145726/1‑61 (MQ=255)
aCGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:1215477/1‑61 (MQ=255)
aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:464856/1‑62 (MQ=255)
aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:4559093/1‑62 (MQ=255)
aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaacca > 1:269497/1‑61 (MQ=255)
aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCATCGCTATTCGCACTCATAaaccaa > 1:804862/1‑62 (MQ=255)
cGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCa < 1:2848979/62‑1 (MQ=255)
cGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGATATTCGCACTCATAAACCAACCTGTCa < 1:1666156/62‑1 (MQ=255)
gCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCa < 1:3148917/61‑1 (MQ=255)
|
GACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCA > W3110S.gb/3459231‑3459337
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
