Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,036,723 |
G→A |
A184T (GCT→ACT) |
ftsY → |
fused Signal Recognition Particle (SRP) receptor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,036,723 | 0 | G | A | 100.0%
| 77.6
/ NA
| 25 | A184T (GCT→ACT) | ftsY | fused Signal Recognition Particle (SRP) receptor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/16); total (9/16) |
GTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCT > W3110S.gb/4036681‑4036781
|
gtggtTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAAcc > 1:1696112/1‑62 (MQ=255)
ttCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGAc > 1:2144990/1‑61 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa < 1:4319798/61‑1 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa < 1:415978/61‑1 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaaa < 1:3886433/61‑1 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa > 1:4575928/1‑60 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa > 1:128452/1‑60 (MQ=255)
tccGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCaa > 1:2155789/1‑60 (MQ=255)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCTGGAAAAACCGACCAAAGAAGGttttt < 1:365526/61‑1 (MQ=39)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:3002434/61‑1 (MQ=255)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:379614/61‑1 (MQ=255)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:3560152/61‑1 (MQ=255)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:1627619/61‑1 (MQ=255)
gaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt < 1:1518712/61‑1 (MQ=255)
gagCAGCCGGTGGAAGAAATCACTCAGGAGCTGGAAAAACCGACCaaa < 1:2636783/48‑1 (MQ=37)
agcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcg < 1:3156905/62‑1 (MQ=255)
agcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcg < 1:1106630/62‑1 (MQ=255)
gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc < 1:1088390/62‑1 (MQ=255)
gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc < 1:3174989/62‑1 (MQ=255)
gcagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc < 1:4089695/62‑1 (MQ=255)
cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg > 1:3723340/1‑62 (MQ=255)
cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg > 1:1366324/1‑62 (MQ=255)
cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgcg > 1:1297718/1‑62 (MQ=255)
cagcCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTcgcgc < 1:1911668/61‑1 (MQ=255)
aTCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCt > 1:3656827/1‑62 (MQ=255)
|
GTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCT > W3110S.gb/4036681‑4036781
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
