Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R2
|
164 |
48.3 |
4710601 |
85.3% |
4018142 |
55.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,123,875 |
G→A |
R255H (CGC→CAC) |
damX → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,123,875 | 0 | G | A | 100.0%
| 210.1
/ NA
| 70 | R255H (CGC→CAC) | damX | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (54/16); total (54/16) |
CATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCGCCCAGCTCGTCAGCAGGCGGTGATTGAACCGAAAAA > W3110S.gb/4123824‑4123911
|
cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc > 1:838746/1‑60 (MQ=255)
cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc > 1:630599/1‑60 (MQ=255)
cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc > 1:628392/1‑60 (MQ=255)
cATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTc > 1:538316/1‑60 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCTCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:1828958/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:3150175/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:4040272/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:4135814/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:1222184/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:1827004/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:1395268/62‑1 (MQ=255)
gcgTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:1465064/62‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:1728329/61‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:285379/61‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:4010600/61‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:213517/61‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:1373443/61‑1 (MQ=255)
gTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagca < 1:1007599/61‑1 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcagcag > 1:4309938/1‑61 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:2097508/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:1012247/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:2917999/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:2964097/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:1101522/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:2446956/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:31851/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:323264/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:3568104/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:640897/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:1799084/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:4518108/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:4614030/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTcag > 1:4619011/1‑58 (MQ=255)
tGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTca > 1:708170/1‑57 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:869423/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:463588/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:808305/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:899747/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2683104/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1124387/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1188855/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1505290/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1512329/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1716272/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1784837/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:1843134/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2089508/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2369644/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2803468/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2604731/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2554073/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAg > 1:2456996/1‑46 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:3591716/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:1211692/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:1383271/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:1431810/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:1572870/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:1772751/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:3186917/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:4309162/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:2433048/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:3258464/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:231941/1‑62 (MQ=255)
acGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGt > 1:3482282/1‑62 (MQ=255)
cGCAAACCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGGACCg < 1:2446568/62‑1 (MQ=255)
aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa > 1:555794/1‑62 (MQ=255)
aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa > 1:1142890/1‑62 (MQ=255)
aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa > 1:1117513/1‑62 (MQ=255)
aCCGCTGAACGTCCGTCCACTACGCACCCAGCTCGTCAGCAGGCGGTGATTGAACCGaaaaa > 1:1115385/1‑62 (MQ=255)
tGAACGTCCGTCCACTACGCACCCAGCTCGTcagc < 1:681137/35‑1 (MQ=255)
|
CATCGCGTGACACGGCGAAAACGCAAACCGCTGAACGTCCGTCCACTACGCGCCCAGCTCGTCAGCAGGCGGTGATTGAACCGAAAAA > W3110S.gb/4123824‑4123911
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
