Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
698,061 |
(GCAC)2→3 |
coding (1539/1665 nt) |
asnB ← |
asparagine synthetase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 698,053 | 1 | . | G | 81.8%
| 27.5
/ 1.1
| 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (9/0); total (9/2) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | W3110S.gb | 698,053 | 2 | . | C | 81.8%
| 25.1
/ 1.4
| 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (9/0); total (9/2) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 |
| * | W3110S.gb | 698,053 | 3 | . | A | 81.8%
| 22.0
/ 1.9
| 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (9/0); total (9/2) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 |
| * | W3110S.gb | 698,053 | 4 | . | C | 81.8%
| 27.7
/ 1.4
| 11 | coding (1547/1665 nt) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (9/0); total (9/2) |
| Fisher's exact test for biased strand distribution p-value = 1.82e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 |
TCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCGGA > W3110S.gb/698015‑698076
||||
tCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCgg < 1:830307/61‑1 (MQ=255)
tCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCgg < 1:86304/61‑1 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCgg > 1:1386072/1‑61 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1091759/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1310596/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:1327613/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:2468288/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:2726761/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:3111558/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:612543/1‑62 (MQ=255)
tttAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACGCACTCAGCGGCGCTCGGa > 1:85570/1‑62 (MQ=255)
||||
TCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCG‑‑‑‑GCACGCACTCAGCGGCGCTCGGA > W3110S.gb/698015‑698076
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
