Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R2 154 32.4 3140800 76.4% 2399571 54.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,687,470 T→C H278R (CAC→CGC)  fumC ← fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,687,4700TC100.0% 37.4 / NA 12H278R (CAC→CGC) fumCfumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/12);  total (0/12)

CATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATC  >  W3110S.gb/1687420‑1687489
                                                  |                   
catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1149732/62‑1 (MQ=255)
catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1389902/62‑1 (MQ=255)
catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1418504/62‑1 (MQ=255)
catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:208791/62‑1 (MQ=255)
catcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:815379/62‑1 (MQ=255)
 atcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAAcc          <  1:1024895/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1048071/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1603157/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:1866573/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:2125734/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:2595866/61‑1 (MQ=255)
         cGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGCGCGCCTGAACCAGGGCATc  <  1:628360/61‑1 (MQ=255)
                                                  |                   
CATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAACGCGCCGTGCGCCTGAACCAGGGCATC  >  W3110S.gb/1687420‑1687489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A