Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,984,357 |
C→T |
L56L (TTG→TTA) |
otsB ← |
trehalose‑6‑phosphate phosphatase, biosynthetic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,984,357 | 0 | C | T | 100.0%
| 69.4
/ NA
| 22 | L56L (TTG→TTA) | otsB | trehalose‑6‑phosphate phosphatase, biosynthetic |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/4); total (18/4) |
GCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAG > W3110S.gb/1984300‑1984401
|
gCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATcaatgctaatg < 1:1692825/62‑1 (MQ=255)
gCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATcaatgctaatg < 1:2397712/62‑1 (MQ=255)
ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc > 1:2804524/1‑62 (MQ=255)
ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc > 1:1759619/1‑62 (MQ=255)
ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc > 1:1768852/1‑62 (MQ=255)
ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc > 1:1115616/1‑62 (MQ=255)
ggTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCTAATGCACCATc > 1:2441819/1‑62 (MQ=255)
ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt < 1:1133535/62‑1 (MQ=255)
ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt < 1:493112/62‑1 (MQ=255)
ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt < 1:1624331/62‑1 (MQ=255)
ttGAGCGCCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCt < 1:25272/62‑1 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:2228492/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:921273/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:682168/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:2717383/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:2379524/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:2034671/1‑61 (MQ=255)
gcCCTGATATCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:1124963/1‑61 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:2356187/1‑54 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:1763543/1‑54 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:1750330/1‑54 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:1531066/1‑54 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:569139/1‑54 (MQ=255)
tatCAATGCTAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg > 1:1187003/1‑54 (MQ=255)
|
GCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAG > W3110S.gb/1984300‑1984401
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
