Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,395,203 |
C→T |
A265T (GCG→ACG) |
nuoN ← |
NADH:ubiquinone oxidoreductase, membrane subunit N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,395,203 | 0 | C | T | 100.0%
| 81.9
/ NA
| 28 | A265T (GCG→ACG) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/23); total (5/23) |
CCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACCAGCA > W3110S.gb/2395160‑2395250
|
ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc > 1:955121/1‑62 (MQ=255)
ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc > 1:1285087/1‑62 (MQ=255)
ccGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGcc > 1:723932/1‑62 (MQ=255)
cAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCaa < 1:1957020/62‑1 (MQ=255)
cAGATAACCGGCCAGGTAAACCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCaa < 1:2389806/62‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:2436058/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:897631/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:840053/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:647468/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:581417/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:571945/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:3029574/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:290733/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:2716073/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:2701037/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:1042966/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:2316485/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:2106372/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:1812548/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:1448602/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:1197640/53‑1 (MQ=255)
aaaCCCCTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:104641/53‑1 (MQ=255)
aaaCCCCTACCGTTTACATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:3041501/53‑1 (MQ=39)
aaCCCCTACCGTTTCCATCGACATCTCGCCGGATTGCAGCGCAATCAGCGCt < 1:2210048/52‑1 (MQ=39)
aaCCCCTACCGTTTCCATCGACATCTCGCCGGATTGCAGCGCAATCAGCGCt < 1:1980114/52‑1 (MQ=39)
ccccTACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCt < 1:1506758/50‑1 (MQ=255)
tACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACcagca > 1:2138068/1‑53 (MQ=255)
tACCGTTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACcagca > 1:743191/1‑53 (MQ=255)
|
CCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACCAGCA > W3110S.gb/2395160‑2395250
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
