Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,923,382 |
(GA)3→4 |
coding (489/660 nt) |
yiaD ← |
predicted outer membrane lipoprotien |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,923,376 | 1 | . | G | 100.0%
| 15.6
/ NA
| 6 | S165? (TCC→TCN) | yiaD | predicted outer membrane lipoprotien |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (6/0); total (6/0) |
| * | W3110S.gb | 3,923,376 | 2 | . | A | 100.0%
| 13.5
/ NA
| 6 | S165S (TCC→TCT) | yiaD | predicted outer membrane lipoprotien |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (6/0); total (6/0) |
CGCTGGCAACGGAATCCGCACGTTGCTGG‑‑GAGAGACGCATGTTCAGGTCGTGACCACCCG > W3110S.gb/3923348‑3923407
||
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:1696365/1‑61 (MQ=255)
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:1905236/1‑61 (MQ=255)
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:746731/1‑61 (MQ=255)
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:2186221/1‑62 (MQ=255)
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:2442417/1‑62 (MQ=255)
cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:780518/1‑62 (MQ=255)
||
CGCTGGCAACGGAATCCGCACGTTGCTGG‑‑GAGAGACGCATGTTCAGGTCGTGACCACCCG > W3110S.gb/3923348‑3923407
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
