Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,931,020 |
C→T |
A360V (GCG→GTG) |
eptB → |
predicted metal dependent hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,931,020 | 0 | C | T | 100.0%
| 145.5
/ NA
| 47 | A360V (GCG→GTG) | eptB | predicted metal dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/28); total (19/28) |
AATGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCGGAGCCACGTAATCGTGGCAA > W3110S.gb/3930961‑3931041
|
aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg > 1:1184686/1‑62 (MQ=255)
aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg > 1:42915/1‑62 (MQ=255)
aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg > 1:2313029/1‑62 (MQ=255)
aaTGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg > 1:2214069/1‑62 (MQ=255)
aaTGTGGGTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTgg > 1:2812457/1‑62 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCa > 1:1042460/1‑58 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCa > 1:2195669/1‑58 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:2969886/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:2980838/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:3029260/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:423437/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:2130997/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:757661/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:1882634/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:1037894/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:1198829/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:1820693/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:1438143/1‑59 (MQ=255)
cTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCAc > 1:834786/1‑59 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:1877062/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:1164398/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:2763416/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:444205/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:681778/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:151054/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:768822/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:2047099/62‑1 (MQ=255)
acGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGTGGCaa < 1:755746/62‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:400951/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:779415/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:3078698/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:863453/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2828256/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:282609/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2806360/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2776734/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2729455/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2627034/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2607760/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:2588607/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:1926922/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:192269/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:1850510/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:175031/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:1471500/39‑1 (MQ=255)
gCTTATCGTGAGCAGATTGGTGTGAAGCCACGTAATCGt < 1:2459647/39‑1 (MQ=38)
aTCGTGAGCAGATTGGTGTGGAGCCACGTAATCGt < 1:1115013/35‑1 (MQ=255)
|
AATGTGGTTCTACAGCAACACGATGGCGGACAACATTGCTTATCGTGAGCAGATTGGTGCGGAGCCACGTAATCGTGGCAA > W3110S.gb/3930961‑3931041
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
