Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,357,180 |
(A)8→9 |
intergenic (+143/‑84) |
yjdJ → / → yjdK |
predicted acyltransferase with acyl‑CoA N‑acyltransferase domain/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,357,172 | 1 | . | A | 100.0%
| 32.7
/ NA
| 12 | intergenic (+135/‑92) | yjdJ/yjdK | predicted acyltransferase with acyl‑CoA N‑acyltransferase domain/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (7/5); total (7/5) |
CACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑AAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGC > W3110S.gb/4357116‑4357227
|
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:3083471/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:30388/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:1382433/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:1415061/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:1746540/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:3019355/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGc < 1:2024286/57‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa < 1:1686952/62‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa < 1:1791503/62‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa < 1:196615/62‑1 (MQ=255)
cacaAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑aaaaa < 1:773753/62‑1 (MQ=255)
gagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCta < 1:1261443/61‑1 (MQ=255)
gagCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCta < 1:2107883/61‑1 (MQ=255)
gTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCTATATgag < 1:1758915/61‑1 (MQ=255)
tGTCCCAAGTCATCCCCTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAatcatc < 1:1032583/61‑1 (MQ=255)
ccTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAg > 1:2087335/1‑62 (MQ=255)
ccTTTACTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAg > 1:716182/1‑62 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGtttt < 1:1179099/60‑1 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg > 1:2624726/1‑61 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTg > 1:1752336/1‑61 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGc > 1:863887/1‑62 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGc > 1:1101056/1‑62 (MQ=255)
cTGAGCAAAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAAAAAGTTTTGc > 1:259575/1‑62 (MQ=255)
|
CACAAAGAATGGGCAGAGAGCGCGTTTTTTTGTCCCAAGTCATCCCCTTTACTGAGC‑AAAAAAAAGAATATCTCCTATATGAGAATCATCAATCGGGGTTAATAAGTTTTGC > W3110S.gb/4357116‑4357227
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
