Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R2
|
154 |
32.4 |
3140800 |
76.4% |
2399571 |
54.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,431,194 |
C→T |
H135H (CAC→CAT) |
rplI → |
50S ribosomal subunit protein L9 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,431,194 | 0 | C | T | 100.0%
| 68.5
/ NA
| 22 | H135H (CAC→CAT) | rplI | 50S ribosomal subunit protein L9 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/3); total (19/3) |
GCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTC > W3110S.gb/4431148‑4431249
|
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGc > 1:875886/1‑50 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:342100/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:328150/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:323989/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:575332/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:592711/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:2822168/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:2696634/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:623174/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:2530830/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:2117455/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:1940366/1‑51 (MQ=255)
gCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCATAGCg > 1:702460/1‑51 (MQ=255)
acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag < 1:2784635/62‑1 (MQ=255)
acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag < 1:102492/62‑1 (MQ=255)
acacGAAGTGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGtagtag < 1:237550/62‑1 (MQ=255)
tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa > 1:282465/1‑62 (MQ=255)
tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa > 1:2942774/1‑62 (MQ=255)
tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa > 1:2916137/1‑62 (MQ=255)
tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaataa > 1:2675999/1‑62 (MQ=255)
tGAGCTTCCAGGTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGaata > 1:1514140/1‑61 (MQ=255)
ggTTCATAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTc > 1:1264321/1‑62 (MQ=255)
|
GCGTTCTGCGTACCACTGGCGAACACGAAGTGAGCTTCCAGGTTCACAGCGAAGTATTCGCGAAAGTGATCGTAAACGTAGTAGCTGAATAATTCGTTATTC > W3110S.gb/4431148‑4431249
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
