Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I1 R2
|
90 |
19.5 |
1203533 |
67.3% |
809977 |
57.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
495,220 |
+C |
coding (73/1041 nt) |
galM ← |
galactose‑1‑epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 495,217 | 1 | . | C | 77.8%
| 74.2
/ 16.1
| 27 | coding (76/1041 nt) | galM | galactose‑1‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (0/6); new base C (0/21); total (0/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
GCACCCCAGTCCATCAGCGTGACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCA > minE/495189‑495270
|
ggaCCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:219435/60‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:4922/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:954141/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:863506/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:802438/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:707635/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:607134/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:583993/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:57251/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:513796/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1064373/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:448577/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:370129/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:257091/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1203343/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1201007/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1167968/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1138992/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1107137/62‑1 (MQ=255)
gCACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:1105724/62‑1 (MQ=255)
cACCCCAGTCCATCAGCGTGACTACCATCCCCTGCGTTGTTACGCAAAGTTAACAGTCGGt < 1:601776/61‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:276161/62‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:503049/62‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:1186683/62‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:64219/62‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:821981/62‑1 (MQ=255)
gACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCa < 1:913384/62‑1 (MQ=255)
|
GCACCCCAGTCCATCAGCGTGACTACCAT‑CCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCA > minE/495189‑495270
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
