Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
421,280 |
G→A |
A400V (GCA→GTA) |
asnB ← |
asparagine synthetase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 421,280 | 0 | G | A | 100.0%
| 186.5
/ NA
| 55 | A400V (GCA→GTA) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/25); total (30/25) |
TGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGC > minE/421225‑421341
|
tgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg > 1:1351183/1‑71 (MQ=255)
tgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg > 1:2206908/1‑71 (MQ=255)
cGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGACCGCGTACTTCCACGCCCCAGGCTGACATCGc > 1:2343719/1‑71 (MQ=255)
gCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt < 1:181198/71‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt < 1:738309/59‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt < 1:1234094/59‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg < 1:2984549/71‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg < 1:1383936/71‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg < 1:819425/71‑1 (MQ=255)
tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg < 1:2351378/71‑1 (MQ=255)
gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTg > 1:476223/1‑58 (MQ=255)
gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt > 1:1606122/1‑71 (MQ=255)
gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgt < 1:1730495/70‑1 (MQ=255)
cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTgg < 1:2061554/71‑1 (MQ=255)
aTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt < 1:1578887/52‑1 (MQ=255)
cAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc > 1:1718886/1‑71 (MQ=255)
ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGc < 1:918592/39‑1 (MQ=255)
ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGc < 1:403464/39‑1 (MQ=255)
ggAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTatgt > 1:751656/1‑60 (MQ=255)
gAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCt > 1:949580/1‑46 (MQ=255)
aaTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGccc > 1:2588396/1‑39 (MQ=255)
aaTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGccc > 1:338820/1‑39 (MQ=255)
aTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAg < 1:2472107/71‑1 (MQ=255)
aTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAg < 1:2877410/71‑1 (MQ=255)
tttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcg > 1:782488/1‑64 (MQ=255)
ttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTca > 1:1754206/1‑71 (MQ=255)
ttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTca > 1:2316026/1‑71 (MQ=255)
tttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCtt > 1:2913622/1‑50 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGTACGCGCGCAGt > 1:1373950/1‑67 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc > 1:77561/1‑60 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc > 1:1453560/1‑60 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgc > 1:1648691/1‑60 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt > 1:1071495/1‑67 (MQ=255)
ttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt > 1:2586233/1‑67 (MQ=255)
tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg > 1:1755888/1‑36 (MQ=255)
tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAgg > 1:1116812/1‑36 (MQ=255)
tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcata < 1:2456893/70‑1 (MQ=255)
tGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcata < 1:2795112/70‑1 (MQ=255)
gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt < 1:1524285/46‑1 (MQ=255)
gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt < 1:2089706/46‑1 (MQ=255)
gTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCt < 1:1611983/46‑1 (MQ=255)
tCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcataca < 1:1969336/70‑1 (MQ=255)
cGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCttt > 1:1446761/1‑46 (MQ=255)
gAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACatat < 1:1004743/71‑1 (MQ=255)
ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg > 1:2346915/1‑70 (MQ=255)
ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc < 1:536999/71‑1 (MQ=255)
ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc < 1:530269/71‑1 (MQ=255)
ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc < 1:2481272/71‑1 (MQ=255)
gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt > 1:635396/1‑45 (MQ=255)
gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt > 1:2806027/1‑45 (MQ=255)
gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt > 1:976398/1‑45 (MQ=255)
gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg > 1:3039536/1‑69 (MQ=255)
gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGc < 1:2619272/70‑1 (MQ=255)
aacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc > 1:1444144/1‑69 (MQ=255)
cgTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCa > 1:1627796/1‑47 (MQ=255)
|
TGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGC > minE/421225‑421341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
