Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,695,639 |
T→C |
E124G (GAG→GGG) |
yfhQ ← |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,695,639 | 0 | T | C | 100.0%
| 131.0
/ NA
| 36 | E124G (GAG→GGG) | yfhQ | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/20); total (16/20) |
TCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCA > minE/1695571‑1695704
|
tCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAAccct > 1:57108/1‑71 (MQ=255)
aGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCAtt > 1:1772447/1‑71 (MQ=255)
aGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCaa < 1:2515871/71‑1 (MQ=255)
aGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCaa < 1:189349/71‑1 (MQ=255)
aGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCaa < 1:1753506/71‑1 (MQ=255)
cGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGc > 1:669515/1‑71 (MQ=255)
gAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGcc < 1:72440/71‑1 (MQ=255)
gCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGa < 1:116321/71‑1 (MQ=255)
cTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGAc < 1:2375290/71‑1 (MQ=255)
tGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGAcg > 1:1081751/1‑71 (MQ=255)
gTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGAc > 1:2195811/1‑71 (MQ=255)
aGCCGCAATCGCGACATGATATTGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2369862/70‑1 (MQ=255)
aGCCGCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCa < 1:2496433/70‑1 (MQ=255)
gCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAacac < 1:1225690/71‑1 (MQ=255)
gCAATCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAacac < 1:1095582/71‑1 (MQ=255)
tCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgc > 1:680865/1‑71 (MQ=255)
tCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgc > 1:1341250/1‑71 (MQ=255)
tCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAgc > 1:2027396/1‑71 (MQ=255)
tCGCGACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGACGACGCGCTCGCGACCAAACACCAgc > 1:1701196/1‑71 (MQ=255)
cgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTc > 1:782666/1‑52 (MQ=255)
cgACATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTc > 1:912293/1‑52 (MQ=255)
aCATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCAc > 1:3073271/1‑71 (MQ=255)
aCATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCAc > 1:1902796/1‑71 (MQ=255)
aCATGATAATGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCAc > 1:179659/1‑71 (MQ=255)
aTGATAATGGCATTTCTGCAACCCTTCATTGGTCa < 1:1332336/35‑1 (MQ=255)
aTGATAATGGCATTTCTGCAACCCTTCATTGGTCa < 1:1147683/35‑1 (MQ=255)
gATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:2041835/1‑35 (MQ=255)
gATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:3164191/1‑35 (MQ=255)
gATAATGGCATTTCTGCAACCCTTCATTGGTCAAg > 1:863556/1‑35 (MQ=255)
aTGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACAcc < 1:2116269/55‑1 (MQ=255)
aTGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:3118679/71‑1 (MQ=255)
aTGGCATTTCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTAt < 1:1346152/71‑1 (MQ=255)
tttCTGCAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGc < 1:2155700/69‑1 (MQ=255)
cAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCa < 1:2739306/44‑1 (MQ=255)
cAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCa < 1:8065/44‑1 (MQ=255)
cAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCtt < 1:2800117/68‑1 (MQ=255)
cAACCCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCa < 1:1276341/70‑1 (MQ=255)
|
TCGCCAGGTTCAGCGAGCTGTATTCCGGGTTAGCCGCAATCGCGACATGATAATGGCATTTCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCA > minE/1695571‑1695704
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
