Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
1,739,943 |
T→C |
intergenic (‑133/+65) |
lepA ← / ← rseC |
GTP binding membrane protein/RseC protein involved in reduction of the SoxR iron‑sulfur cluster |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,739,943 | 0 | T | C | 100.0%
| 216.6
/ NA
| 59 | intergenic (‑133/+65) | lepA/rseC | GTP binding membrane protein/RseC protein involved in reduction of the SoxR iron‑sulfur cluster |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/27); total (32/27) |
AACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAAT > minE/1739876‑1740008
|
aaCGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATg > 1:3159570/1‑71 (MQ=255)
aaCGTCTGTATTAATAGAAACGCCGAATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATg > 1:1314456/1‑71 (MQ=255)
cGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGa > 1:94273/1‑71 (MQ=255)
cGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGa > 1:2346149/1‑71 (MQ=255)
tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATtg < 1:2486507/68‑1 (MQ=255)
tATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTgg > 1:1405857/1‑71 (MQ=255)
aTTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTgg > 1:1177708/1‑70 (MQ=255)
aTTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTgg > 1:248528/1‑70 (MQ=255)
ttAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTa < 1:938749/71‑1 (MQ=255)
aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc < 1:1404854/70‑1 (MQ=255)
aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc < 1:3187348/70‑1 (MQ=255)
aTAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc < 1:1791393/70‑1 (MQ=255)
aGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATtgt < 1:1425978/62‑1 (MQ=255)
aaaCGCCGCATTCTACACTACAACATTGAGGCGAGTAAATGTTCATACCGCATGGATTGTgg > 1:2539519/1‑62 (MQ=255)
aaaCGCCGCATTCTACACTACAACATTGAGGAGAGGAAATGTTCATACCGCATGGATTGTgg > 1:187916/1‑62 (MQ=255)
cgcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTcc < 1:3187000/71‑1 (MQ=255)
gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAAc < 1:1869849/66‑1 (MQ=255)
gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCt > 1:2115667/1‑71 (MQ=255)
gcATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCt > 1:2536234/1‑71 (MQ=255)
aTTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGt > 1:3149429/1‑66 (MQ=255)
ttCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCg > 1:3056112/1‑70 (MQ=255)
ttCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCg > 1:93204/1‑70 (MQ=255)
ctacactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt > 1:559236/1‑71 (MQ=255)
cactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAtt < 1:336597/69‑1 (MQ=255)
cactacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:265086/68‑1 (MQ=255)
actacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGt > 1:3019288/1‑71 (MQ=255)
actacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGt > 1:1635028/1‑71 (MQ=255)
actacaACATTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGt < 1:1430383/71‑1 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:1789607/1‑46 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:2795365/1‑46 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:1331977/1‑46 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:1340894/1‑46 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:1309220/1‑46 (MQ=255)
caacaTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATc > 1:794817/1‑46 (MQ=255)
caTTGAGGCGAGGAAATGTTCATAGCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:2753120/60‑1 (MQ=255)
aTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaaa < 1:1938219/71‑1 (MQ=255)
aTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaa > 1:2779871/1‑70 (MQ=255)
aTTGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaa > 1:1166269/1‑70 (MQ=255)
ttGAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCaaaa < 1:1428199/71‑1 (MQ=255)
gAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAAt < 1:636128/70‑1 (MQ=255)
gAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATg > 1:167109/1‑71 (MQ=255)
gAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATg > 1:2800428/1‑71 (MQ=255)
gAGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATg > 1:707097/1‑71 (MQ=255)
aGGCGAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGc < 1:2866418/71‑1 (MQ=255)
gAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGGAACGTCCTCGCATTTGTTATGCAAAATGcaac > 1:1416246/1‑70 (MQ=255)
gAGGAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaaca < 1:1955616/71‑1 (MQ=255)
ggAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCa < 1:822476/59‑1 (MQ=255)
gAAATGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaaca < 1:2652879/68‑1 (MQ=255)
tGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaac < 1:2310213/63‑1 (MQ=255)
tGTTCATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGcaac < 1:1854910/63‑1 (MQ=255)
cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGa > 1:319531/1‑70 (MQ=255)
cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGa > 1:3457/1‑70 (MQ=255)
cATACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGa > 1:1321878/1‑70 (MQ=255)
tACCGCATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAAt < 1:2608947/71‑1 (MQ=255)
aCCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:2804463/37‑1 (MQ=255)
aCCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:377392/37‑1 (MQ=255)
aCCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:740559/37‑1 (MQ=255)
aCCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:220171/37‑1 (MQ=255)
aCCGCATGGATTGTGGTATCTGGAAACGTCCTCGCAt < 1:1454862/37‑1 (MQ=255)
|
AACGTCTGTATTAATAGAAACGCCGCATTCTACACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAAT > minE/1739876‑1740008
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
