Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I2 R2
|
64 |
60.0 |
3197630 |
92.7% |
2964203 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
2,736,671 |
T→C |
F605F (TTT→TTC) |
fusA → |
protein chain elongation factor EF‑G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,736,671 | 0 | T | C | 100.0%
| 141.6
/ NA
| 39 | F605F (TTT→TTC) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/26); total (13/26) |
ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAG > minE/2736604‑2736734
|
aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCaa < 1:546476/70‑1 (MQ=255)
aTGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGaaa < 1:2090159/71‑1 (MQ=255)
cGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa < 1:536203/71‑1 (MQ=255)
ttGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaaa < 1:2885207/71‑1 (MQ=255)
cctcTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGtt > 1:2133660/1‑71 (MQ=255)
tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCtac > 1:2644415/1‑69 (MQ=255)
tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTg > 1:2039481/1‑70 (MQ=255)
cgAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCtac > 1:2200858/2‑69 (MQ=255)
aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCtt < 1:853687/71‑1 (MQ=255)
tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCaa < 1:3001750/46‑1 (MQ=255)
ggCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGaaa > 1:1288268/1‑55 (MQ=255)
aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa < 1:3028631/46‑1 (MQ=255)
aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa < 1:2992821/46‑1 (MQ=255)
aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa < 1:2316274/46‑1 (MQ=255)
aaaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa < 1:2311045/46‑1 (MQ=255)
aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:1228349/71‑1 (MQ=255)
aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg > 1:1056289/1‑70 (MQ=255)
aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg > 1:377369/1‑70 (MQ=255)
ggCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAgg > 1:3163419/1‑71 (MQ=255)
ctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg < 1:1601461/69‑1 (MQ=255)
ctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg > 1:59572/1‑69 (MQ=255)
tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGaa < 1:734030/71‑1 (MQ=255)
tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGaa < 1:490163/71‑1 (MQ=255)
tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa > 1:2069227/1‑70 (MQ=255)
tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:496333/70‑1 (MQ=255)
tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:426229/70‑1 (MQ=255)
gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:1701604/54‑1 (MQ=255)
gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:1329583/54‑1 (MQ=255)
gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:404590/54‑1 (MQ=255)
ccTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt < 1:2569388/71‑1 (MQ=255)
ccTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt < 1:1893577/71‑1 (MQ=255)
cTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTc < 1:2629935/71‑1 (MQ=255)
tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg < 1:520441/58‑1 (MQ=255)
tttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg < 1:1401852/58‑1 (MQ=255)
tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCg > 1:2426934/1‑70 (MQ=255)
aagaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa < 1:1465024/71‑1 (MQ=255)
agaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCgg > 1:1927483/1‑68 (MQ=255)
gaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa > 1:3050731/1‑69 (MQ=255)
agGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAagag < 1:1417347/70‑1 (MQ=255)
|
ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAG > minE/2736604‑2736734
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
