Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R2
|
24 |
47.7 |
2431847 |
96.1% |
2337004 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
340,151 |
T→A |
A14A (GCT→GCA) |
ybaS → |
predicted glutaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 340,151 | 0 | T | A | 97.1%
| 216.2
/ ‑1.4
| 70 | A14A (GCT→GCA) | ybaS | predicted glutaminase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (30/37); total (30/40) |
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 |
GCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGT > minE/340083‑340216
|
gCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATa < 1:343233/71‑1 (MQ=255)
tataAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACAcc > 1:443006/1‑70 (MQ=255)
taAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCaa < 1:369798/71‑1 (MQ=255)
aaaaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCaa < 1:1683611/70‑1 (MQ=255)
aaaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAtt < 1:2341361/71‑1 (MQ=255)
aaaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAtt > 1:2184395/1‑71 (MQ=255)
aaaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAtt > 1:1868754/1‑71 (MQ=255)
aaaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAtt > 1:157001/1‑71 (MQ=255)
aaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATa > 1:441423/1‑61 (MQ=255)
aaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAttt < 1:40438/71‑1 (MQ=255)
aaaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAttt < 1:1412503/71‑1 (MQ=255)
aaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAttt > 1:336791/1‑70 (MQ=255)
aaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAttt > 1:1768325/1‑70 (MQ=255)
aaGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAtt > 1:1550576/1‑69 (MQ=255)
aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtca < 1:1015793/71‑1 (MQ=255)
aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtca < 1:1013027/71‑1 (MQ=255)
aGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtca < 1:1132104/71‑1 (MQ=255)
aGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactc < 1:1623408/71‑1 (MQ=255)
aGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcact > 1:1915172/1‑70 (MQ=255)
ttAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATa < 1:1540/54‑1 (MQ=255)
tAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAAt < 1:2192445/61‑1 (MQ=255)
aCAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATa < 1:740254/51‑1 (MQ=255)
aCAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTa < 1:1267551/71‑1 (MQ=255)
aaaGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAAcg < 1:119074/71‑1 (MQ=255)
aaGATGTTAGATGCAAACAAATTAGAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAAcgg > 1:993672/1‑71 (MQ=255)
aaGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAAcgg < 1:376260/71‑1 (MQ=255)
tAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTaa > 1:2205008/1‑61 (MQ=255)
tAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACa > 1:2247967/1‑70 (MQ=255)
tAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACa > 1:144812/1‑70 (MQ=255)
aGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACaa < 1:2312332/70‑1 (MQ=255)
aGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACaa < 1:32697/70‑1 (MQ=255)
aGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACaa < 1:2383499/70‑1 (MQ=255)
aGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACaa > 1:1890908/1‑70 (MQ=255)
aTGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAAt < 1:1738086/71‑1 (MQ=255)
tGCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATg > 1:1459394/1‑71 (MQ=255)
gCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactcac > 1:1948576/1‑52 (MQ=255)
gCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactcac > 1:2275047/1‑52 (MQ=255)
gCAAACAAATTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAAt > 1:1099029/1‑69 (MQ=255)
caaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACaa < 1:294972/65‑1 (MQ=255)
caaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGcc < 1:1920852/71‑1 (MQ=255)
caaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGcc < 1:1670577/71‑1 (MQ=255)
aaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAAcggcg < 1:25212/59‑1 (MQ=255)
aaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGcc > 1:218568/1‑70 (MQ=255)
aaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCg < 1:2194315/71‑1 (MQ=255)
aaacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCg < 1:648880/71‑1 (MQ=255)
aacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGa > 1:236790/1‑60 (MQ=255)
aacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGa > 1:602595/1‑60 (MQ=255)
aacaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGa < 1:987522/71‑1 (MQ=255)
acaaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACaaaa > 1:1265354/1‑64 (MQ=255)
caaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGAt > 1:344891/1‑70 (MQ=255)
caaaATACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGAt > 1:928817/1‑70 (MQ=255)
aaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactca > 1:1920484/1‑45 (MQ=255)
aaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactca > 1:1430975/1‑45 (MQ=255)
aaaTTACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAAc < 1:866376/51‑1 (MQ=255)
aTTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactc < 1:2235131/42‑1 (MQ=255)
aTTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactc < 1:1613855/42‑1 (MQ=255)
aTTACAGCAGGCAGTGGATCAGGCATACACCCAATTtcactc < 1:1410291/42‑1 (MQ=255)
ttACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACAt < 1:1969092/71‑1 (MQ=255)
ttACAGCAGGCAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACAt < 1:984985/71‑1 (MQ=255)
tACAGCTGGGAGTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCg < 1:709587/63‑1 (MQ=255)
cagTGGATCAGGCCTACACCCAATTTCACTCACTTAACGGCGGACaa < 1:1433817/47‑1 (MQ=255)
agTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTgg < 1:1291949/71‑1 (MQ=255)
gTGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACa < 1:1873426/44‑1 (MQ=255)
tGGATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCg < 1:522844/71‑1 (MQ=255)
gATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTc > 1:1346060/1‑64 (MQ=255)
gATCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGaa > 1:1907697/1‑71 (MQ=255)
aTCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGaa > 1:2247545/1‑70 (MQ=255)
aTCAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGa < 1:314954/69‑1 (MQ=255)
cAGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGAtt > 1:203461/1‑49 (MQ=255)
aGGCATACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGt < 1:1959436/70‑1 (MQ=255)
|
GCATCTATAAAAAGGAGTTAACAAAAGATGTTAGATGCAAACAAATTACAGCAGGCAGTGGATCAGGCTTACACCCAATTTCACTCACTTAACGGCGGACAAAATGCCGATTACATTCCCTTTCTGGCGAATGT > minE/340083‑340216
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
