breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 61,929 G→A F510F (TTC→TTT polB ← DNA polymerase II
RA 86,263 C→T S253S (AGC→AGT murC → UDP‑N‑acetylmuramate:L‑alanine ligase
RA 204,511 C→T intergenic (+111/‑73) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 204,556 A→G intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 340,151 T→A A14A (GCT→GCA ybaS → predicted glutaminase
RA 498,954 C→T Q430Q (CAG→CAA modF ← fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily
RA 694,703 G→A G89S (GGC→AGC)  appC → cytochrome bd‑II oxidase, subunit I
RA 817,564 C→T L113L (CTC→CTT prfA → peptide chain release factor RF‑1
RA 894,732 C→T D445N (GAC→AAC)  sapA ← predicted antimicrobial peptide transporter subunit
RA 942,255 C→T R296K (AGG→AAG)  ddpF ← D‑Ala‑D‑Ala transporter subunit
RA 1,017,691 A→G E50G (GAA→GGA)  ydgA → conserved hypothetical protein
RA 1,036,505 G→A A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein
RA 1,291,205 C→T intergenic (+320/+133) yeeN → / ← asnW conserved hypothetical protein/tRNA‑Asn
RA 1,560,516 C→T P175S (CCA→TCA)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA
RA 1,892,019 +GC intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein
RA 2,019,887 C→T Q169* (CAA→TAA)  dkgA → 2,5‑diketo‑D‑gluconate reductase A
RA 2,078,255 C→T D88N (GAT→AAT)  uxaA ← altronate hydrolase
RA 2,281,038 G→A Q95* (CAG→TAG)  fabR ← DNA‑binding transcriptional repressor
RA 2,464,298 G→A Q115Q (CAG→CAA atpI → ATP synthase, membrane‑bound accesory subunit
RA 2,489,124 G→A T41T (ACG→ACA dnaN → DNA polymerase III, beta subunit
RA 2,520,434 G→A W44* (TGG→TAG)  ligB → DNA ligase, NAD(+)‑dependent
RA 2,881,808 C→T V87I (GTT→ATT)  ytfG ← NAD(P)H:quinone oxidoreductase
RA 2,891,553 A→G K70E (AAA→GAA)  ytfQ → predicted sugar transporter subunit
RA 2,910,326 (A)6→7 coding (1318/2697 nt) mgtA → magnesium transporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? minE 206882 =NA (NA)14 (0.330) 6/110 2.9 NA noncoding (2299/2904 nt) rrlH 23S ribosomal RNA
?minE 206901 = NA (NA)noncoding (2318/2904 nt) rrlH 23S ribosomal RNA
* ? minE 1611749 =7 (0.150)13 (0.310)
+ATGCGGT
7/106 2.5 30.3% coding (1404/1404 nt) eutA reactivating factor for ethanolamine ammonia lyase
?minE = 2603083 61 (1.300)coding (684/753 nt) yhiQ predicted SAM‑dependent methyltransferase