Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F28 I3 R2
|
24 |
47.7 |
2431847 |
96.1% |
2337004 |
60.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
minE |
498,954 |
C→T |
Q430Q (CAG→CAA) |
modF ← |
fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 498,954 | 0 | C | T | 93.4%
| 186.6
/ 3.9
| 61 | Q430Q (CAG→CAA) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (24/33); total (25/36) |
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 |
GTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGC > minE/498886‑499016
|
gTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTg < 1:591563/71‑1 (MQ=255)
tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCATCGGATCAAGCCCTTGt < 1:2280382/71‑1 (MQ=255)
tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTg < 1:2376055/70‑1 (MQ=255)
tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGt < 1:459024/71‑1 (MQ=255)
tCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAg > 1:1124280/1‑71 (MQ=255)
cACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAg < 1:917083/70‑1 (MQ=255)
cACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGt > 1:59564/1‑71 (MQ=255)
aCCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGt < 1:2396725/70‑1 (MQ=255)
ttCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGtt > 1:2394701/1‑71 (MQ=255)
gCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCa < 1:1745639/70‑1 (MQ=255)
gCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCAt < 1:222156/71‑1 (MQ=255)
aaTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc > 1:305181/1‑69 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc > 1:61081/1‑68 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:127925/70‑1 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:125066/70‑1 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCga < 1:1985769/70‑1 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCg < 1:1949386/69‑1 (MQ=255)
tCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCgaga < 1:2105326/71‑1 (MQ=255)
aGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGaa > 1:221492/1‑70 (MQ=255)
aGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAAt < 1:1679124/71‑1 (MQ=255)
aTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAg > 1:1482065/1‑69 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAAt < 1:150089/65‑1 (MQ=255)
acaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:219365/71‑1 (MQ=255)
aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCaa > 1:1636075/1‑65 (MQ=255)
aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCgg < 1:919783/71‑1 (MQ=255)
aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:170521/50‑1 (MQ=255)
aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:415472/50‑1 (MQ=255)
aCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCAt < 1:2201913/50‑1 (MQ=255)
ggcggATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCg > 1:1114652/1‑50 (MQ=255)
gcggATAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGtt < 1:2159382/71‑1 (MQ=255)
aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2361019/1‑71 (MQ=255)
aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2291874/1‑71 (MQ=255)
aTAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:1036353/1‑71 (MQ=255)
tAAGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:2064602/1‑70 (MQ=255)
aGCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATc < 1:1842507/41‑1 (MQ=255)
gCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCgaga < 1:228287/44‑1 (MQ=255)
gCTGGCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCg < 1:250471/57‑1 (MQ=255)
ggCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:418092/52‑1 (MQ=255)
ggCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt < 1:237503/52‑1 (MQ=255)
gCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgcg < 1:2038997/70‑1 (MQ=255)
gCGATTCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgc < 1:1980505/69‑1 (MQ=255)
gCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATaa < 1:1999394/44‑1 (MQ=255)
gATTCAGCGGATCAACCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:6447/1‑44 (MQ=255)
ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt > 1:184186/1‑47 (MQ=255)
ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGt > 1:2316306/1‑47 (MQ=255)
ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAg < 1:834618/62‑1 (MQ=255)
ttCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAg < 1:1151220/62‑1 (MQ=255)
tCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGttt > 1:410612/1‑55 (MQ=255)
tCAGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGttt > 1:554907/1‑55 (MQ=255)
aGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACaa < 1:2193431/70‑1 (MQ=255)
aGCGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt < 1:2208218/71‑1 (MQ=255)
cGGATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt > 1:993457/1‑69 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGAt > 1:1901162/1‑46 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCa > 1:1429030/1‑52 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCAc > 1:776653/1‑53 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACa > 1:485663/1‑66 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAAt > 1:750571/1‑68 (MQ=255)
ggATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAg < 1:2065259/71‑1 (MQ=255)
gATCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAg > 1:1052617/1‑70 (MQ=255)
tCAAGCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAgcgc < 1:1458559/71‑1 (MQ=255)
gCCCTTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTgcgc < 1:1053787/54‑1 (MQ=255)
|
GTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGC > minE/498886‑499016
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
