Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,020,867 |
A→G |
68.6% |
S159P (TCC→CCC) |
sulA ← |
SOS cell division inhibitor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,020,867 | 0 | A | G | 68.6%
| 42.4
/ 25.4
| 35 | S159P (TCC→CCC) | sulA | SOS cell division inhibitor |
| Reads supporting (aligned to +/- strand): ref base A (5/6); new base G (22/2); total (27/8) |
| Fisher's exact test for biased strand distribution p-value = 5.76e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 |
GGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT > W3110S.gb/1020813‑1020927
|
ggATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGc > 1:2984401/1‑70 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:1270929/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:3222911/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2968204/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2757073/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:735339/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:2135137/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:1822152/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:928097/1‑56 (MQ=255)
aaTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGGAAGTTg > 1:788056/1‑56 (MQ=255)
aTGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa < 1:778636/70‑1 (MQ=255)
aTGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGa < 1:2204706/70‑1 (MQ=255)
agTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcacc < 1:1863626/71‑1 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAcccc > 1:1824933/1‑70 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2359054/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:3004882/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:1647052/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:646407/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:739519/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2148503/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:2061252/1‑71 (MQ=255)
gTGAATTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCa > 1:1900445/1‑71 (MQ=255)
aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:772814/1‑71 (MQ=255)
aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:1054178/1‑71 (MQ=255)
aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:930950/1‑71 (MQ=255)
aaTTTTTAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAg > 1:2871654/1‑71 (MQ=255)
ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATaaa > 1:3149969/1‑60 (MQ=255)
ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:312655/1‑71 (MQ=255)
ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:1784598/1‑71 (MQ=255)
ttttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGt > 1:1695531/1‑71 (MQ=255)
ttAGCCCGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:2097026/70‑1 (MQ=255)
ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:2030949/70‑1 (MQ=255)
ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGtt < 1:1389853/70‑1 (MQ=255)
ttAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCGTACCGGACGCATAATAAACCCCATAGCGtt < 1:2658110/70‑1 (MQ=255)
cccGGGAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCataata < 1:2636658/52‑1 (MQ=255)
|
GGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTT > W3110S.gb/1020813‑1020927
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
