Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
297,472 |
+T |
coding (479/957 nt) |
yagQ ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 297,469 | 1 | . | T | 100.0%
| 18.6
/ NA
| 8 | T161N (ACC→AAC) | yagQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (0/8); total (0/8) |
GATTCCAGCCCGTCCGGG‑TTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGC > W3110S.gb/297452‑297514
|
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:1027161/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:1478402/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:1925432/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:2369678/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:355589/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:431074/64‑1 (MQ=255)
gaTTCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:664171/64‑1 (MQ=255)
ttCCAGCCCGTCCGGGTTTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAgc < 1:1746960/62‑1 (MQ=255)
|
GATTCCAGCCCGTCCGGG‑TTTGCGTGGGCAGGCACACCAGCGATTGTGCTTGCGGATCGTAGC > W3110S.gb/297452‑297514
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
