Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,609,975 |
C→T |
L254L (CTG→CTA) |
yneE ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,609,975 | 0 | C | T | 100.0%
| 11.8
/ NA
| 5 | L254L (CTG→CTA) | yneE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (5/0); total (5/0) |
AATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAA > W3110S.gb/1609932‑1609996
|
aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:1956868/1‑65 (MQ=255)
aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:1973486/1‑65 (MQ=255)
aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:2224614/1‑65 (MQ=255)
aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:763388/1‑65 (MQ=255)
aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATaaaa > 1:76707/1‑65 (MQ=255)
|
AATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAA > W3110S.gb/1609932‑1609996
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
