Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I2 R1 330 0.0 2544031 91.9% 2337964 62.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,841,701 C→T L134L (CTG→CTA)  hycH ← protein required for maturation of hydrogenase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,841,7010CT100.0% 220.1 / NA 63L134L (CTG→CTA) hycHprotein required for maturation of hydrogenase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/28);  total (35/28)

CCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGA  >  W3110S.gb/2841659‑2841740
                                          |                                       
cccATCATGCTATTGCGAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1210665/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAg                                       >  1:1196535/1‑45 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:529101/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1898441/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2041134/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:211455/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2147893/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2160361/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2211398/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2216041/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:2437409/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:424872/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:496916/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1065610/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:538657/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:591490/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:64700/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:650856/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:693864/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:733993/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:759978/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:85268/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1162055/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1205580/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:135845/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1162119/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:137931/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1495523/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1546066/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1120097/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1628155/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1259208/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGa                   >  1:1226695/1‑65 (MQ=255)
cccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTAACGTAGTCTCCTCACCATCAAATAGa                   >  1:2465327/1‑65 (MQ=255)
         cTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGtttt          >  1:510742/1‑65 (MQ=255)
                 gacacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:332844/64‑1 (MQ=255)
                 aaCACAGAGTAAAACTTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:788970/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:578873/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:620464/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:505804/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1186293/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:674964/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:718754/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:722981/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1017119/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:905800/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:981186/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:56643/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:542043/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1238031/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1864908/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1359148/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:362619/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:300640/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2498332/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2428594/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:139495/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1404116/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1612160/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:2083984/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1992409/65‑1 (MQ=255)
                 aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1680879/65‑1 (MQ=255)
                  acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatga  <  1:1959218/64‑1 (MQ=255)
                                          |                                       
CCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGA  >  W3110S.gb/2841659‑2841740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A