Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,841,701 |
C→T |
L134L (CTG→CTA) |
hycH ← |
protein required for maturation of hydrogenase 3 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,841,701 | 0 | C | T | 100.0%
| 161.0
/ NA
| 48 | L134L (CTG→CTA) | hycH | protein required for maturation of hydrogenase 3 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/18); total (30/18) |
CCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTG > W3110S.gb/2841650‑2841736
|
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:886744/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:4514/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:474310/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:57258/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:290176/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2694301/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2682350/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2367601/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2353425/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2211654/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:717993/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2096499/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:782310/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:868511/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:1509658/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:1482572/64‑1 (MQ=255)
ccATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:140344/64‑1 (MQ=255)
tcatcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCa < 1:2996417/65‑1 (MQ=255)
tATTGCCAACACAGAGTAAATCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTc > 1:36268/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGAt > 1:2558921/1‑53 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg > 1:1195750/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg > 1:1368714/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg > 1:101753/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg > 1:2619873/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtg > 1:3002878/1‑65 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt > 1:2426666/1‑64 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt > 1:2329734/1‑64 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt > 1:2031953/1‑64 (MQ=255)
tGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTAACCATCAAATAGATGGCGTTTTCCtg > 1:119670/1‑65 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2660218/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:584676/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:607178/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:672311/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:854191/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:749948/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:471942/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:3010531/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2960155/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2842110/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2618609/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2317784/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:2134655/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:210258/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:1963578/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:1746156/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:1149627/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATa > 1:1131652/1‑45 (MQ=255)
acacAGAGTAAAACGTCAGTCACGTAGACTCCTCACCATCAAATa > 1:873043/1‑45 (MQ=255)
|
CCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTG > W3110S.gb/2841650‑2841736
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
