Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,504,714 |
G→A |
F711F (TTC→TTT) |
metL ← |
fused aspartokinase II and homoserine dehydrogenase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,504,714 | 0 | G | A | 100.0%
| 21.6
/ NA
| 8 | F711F (TTC→TTT) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/8); total (0/8) |
GCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCT > W3110S.gb/3504671‑3504735
|
gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:1619910/65‑1 (MQ=255)
gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:1622847/65‑1 (MQ=255)
gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2040667/65‑1 (MQ=255)
gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:204311/65‑1 (MQ=255)
gCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2141352/65‑1 (MQ=255)
gCCGGTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2043867/65‑1 (MQ=255)
ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAACTGGGCGATGCTGCCGCCt < 1:664706/56‑1 (MQ=255)
cGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt < 1:2446430/35‑1 (MQ=255)
|
GCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCT > W3110S.gb/3504671‑3504735
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
