Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,504,714 |
G→A |
F711F (TTC→TTT) |
metL ← |
fused aspartokinase II and homoserine dehydrogenase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,504,714 | 0 | G | A | 100.0%
| 199.1
/ NA
| 67 | F711F (TTC→TTT) | metL | fused aspartokinase II and homoserine dehydrogenase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (41/26); total (41/26) |
CCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCG > W3110S.gb/3504648‑3504778
|
ccccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAAt < 1:2111321/71‑1 (MQ=255)
ccATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg < 1:2655226/71‑1 (MQ=255)
aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:217226/71‑1 (MQ=255)
aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:4645533/71‑1 (MQ=255)
aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:1353242/71‑1 (MQ=255)
aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:331720/71‑1 (MQ=255)
aTTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:3775602/71‑1 (MQ=255)
ttCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa > 1:1790077/1‑71 (MQ=255)
tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATgg > 1:3849873/1‑66 (MQ=255)
tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGa < 1:4298395/70‑1 (MQ=255)
tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt > 1:893934/1‑71 (MQ=255)
tCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt > 1:512991/1‑71 (MQ=255)
cgcgGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt < 1:1708776/70‑1 (MQ=255)
cgcgGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAt < 1:1583230/70‑1 (MQ=255)
cGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTgccgc < 1:1186134/71‑1 (MQ=255)
tCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAAt > 1:3690310/1‑52 (MQ=255)
ttGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTc < 1:300773/48‑1 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt > 1:3931672/1‑45 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt > 1:4462403/1‑45 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGt > 1:3117407/1‑45 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCaa > 1:1065112/1‑65 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg > 1:2697191/1‑69 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg > 1:1608441/1‑69 (MQ=255)
gCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAg > 1:2203807/1‑69 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:2350175/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:47945/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:4552774/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:4434739/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:4014094/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:3139886/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:3390661/71‑1 (MQ=255)
aCCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg < 1:3261570/71‑1 (MQ=255)
ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg > 1:2707728/1‑70 (MQ=255)
ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAgg > 1:3818583/1‑70 (MQ=255)
ccATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGc < 1:1129815/71‑1 (MQ=255)
cATCTGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa > 1:2436605/1‑71 (MQ=255)
atatGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa > 1:664203/4‑70 (MQ=255)
tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGAtgctg > 1:3797487/1‑45 (MQ=255)
tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCt > 1:4510883/1‑51 (MQ=255)
tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg > 1:505840/1‑71 (MQ=255)
tGCTCGTTCAGTTCATCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAg > 1:3903085/1‑71 (MQ=255)
gttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGa < 1:3019727/69‑1 (MQ=255)
ttcagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTc < 1:2744751/71‑1 (MQ=255)
cagttcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca > 1:2285662/1‑71 (MQ=255)
tcaTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca > 1:2012618/1‑67 (MQ=255)
caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGa > 1:4211163/1‑47 (MQ=255)
caTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCca > 1:549105/1‑66 (MQ=255)
aTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGt < 1:3154037/70‑1 (MQ=255)
aTCGCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTa < 1:2865106/71‑1 (MQ=255)
gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTcc > 1:325655/1‑61 (MQ=255)
gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCt > 1:1643083/1‑71 (MQ=255)
gCCATTTTCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCt > 1:103451/1‑71 (MQ=255)
aTTTTCAAAAAAATGGTAGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGAt > 1:1999809/1‑71 (MQ=255)
ttttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTc < 1:2829768/56‑1 (MQ=255)
ttCGAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:2883823/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGTTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:1206538/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGGCGCCTTCGCAATGAGCAGGCACCAGCGATTCca > 1:3368348/1‑56 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGc > 1:4559648/1‑39 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCa > 1:1800960/1‑44 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGCTTCCACACGTACCTGATCCg > 1:874161/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGAtt > 1:3785706/1‑53 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:4276541/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:1698868/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:171266/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:513580/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:1931950/1‑71 (MQ=255)
ttCAAAAAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCg > 1:780669/1‑71 (MQ=255)
|
CCCCATTTCGCGGGCCGCTTCCAGCCGTTGCACCATCTGCTCGTTCAGTTCATCGCCATTTTCAAAGAAATGGTCGATGCTGCCGCCTTCGCAATGAGCAGGCACCAGCGATTCCACACGTACCTGATCCG > W3110S.gb/3504648‑3504778
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
