Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I2 R1 330 0.0 2544031 91.9% 2337964 62.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,777,889 T→C V359A (GTG→GCG)  glvC → arbutin specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,777,8890TC94.4% 57.7 / ‑5.6 18V359A (GTG→GCG) glvCarbutin specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/17);  minor base G (0/1);  total (0/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAG  >  W3110S.gb/3777842‑3777906
                                               |                 
cttGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1953146/63‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGGGGGCAACATGGGCGGAg  <  1:1900227/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1927813/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:915796/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:702803/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:433719/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:334046/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:2430392/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:2168131/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:2028668/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1252843/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1862369/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1566566/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1300455/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1295254/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1283508/65‑1 (MQ=255)
cGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1281883/65‑1 (MQ=255)
 gTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGCGGGCAACATGGGCGGAg  <  1:1762756/64‑1 (MQ=255)
                                               |                 
CGTGCTGGCGGCCTCAATGTCGACCGTAATGTATCTCTTTGGTGTGGTGGGCAACATGGGCGGAG  >  W3110S.gb/3777842‑3777906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A