Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,575,785 |
C→T |
A378T (GCT→ACT) |
pqqL ← |
predicted peptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,575,785 | 0 | C | T | 100.0%
| 68.2
/ NA
| 23 | A378T (GCT→ACT) | pqqL | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/23); total (0/23) |
GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCC > W3110S.gb/1575725‑1575843
|
gATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaa < 1:2090233/65‑1 (MQ=255)
ttGACATCATCGTGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:2567255/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:1477775/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:958661/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:95487/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:596642/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:560800/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:459151/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:3025495/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:2947027/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:2029394/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:1793583/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:1766562/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:1258164/65‑1 (MQ=255)
ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCAATTaa < 1:2978043/65‑1 (MQ=255)
tGACATCATCGAGTTCTTCAGTAGGAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:2959662/64‑1 (MQ=255)
tGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:2724307/64‑1 (MQ=255)
tGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTaa < 1:821040/64‑1 (MQ=255)
cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc < 1:1850800/65‑1 (MQ=255)
cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc < 1:651947/65‑1 (MQ=255)
cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc < 1:658486/65‑1 (MQ=255)
cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc < 1:827466/65‑1 (MQ=255)
cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATcc < 1:162686/65‑1 (MQ=255)
|
GATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCC > W3110S.gb/1575725‑1575843
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
