Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,919,937 |
+C |
coding (714/2634 nt) |
yebT → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,919,937 | 1 | . | C | 100.0%
| 15.8
/ NA
| 6 | V238V (GTG→GTC) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/6); total (0/6) |
CGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGG > W3110S.gb/1919898‑1919969
|
cGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt < 1:2578982/64‑1 (MQ=255)
cGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt < 1:2784009/64‑1 (MQ=255)
ggCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt < 1:995447/63‑1 (MQ=255)
tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg < 1:1275108/64‑1 (MQ=255)
tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg < 1:1433602/64‑1 (MQ=255)
tGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACgg < 1:3074272/64‑1 (MQ=255)
|
CGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGG > W3110S.gb/1919898‑1919969
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
