Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,919,937 |
+C |
coding (714/2634 nt) |
yebT → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,919,937 | 1 | . | C | 100.0%
| 117.1
/ NA
| 34 | V238V (GTG→GTC) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (22/12); total (22/12) |
GAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCG > W3110S.gb/1919868‑1920000
|
gAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTg > 1:1833339/1‑70 (MQ=255)
aaaaaaGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTgca < 1:3992195/71‑3 (MQ=255)
gTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTgg > 1:3672989/1‑70 (MQ=255)
tAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGa < 1:3349669/70‑1 (MQ=255)
tCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggc > 1:2736078/1‑71 (MQ=255)
cTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggcg > 1:3630128/1‑71 (MQ=255)
gAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCTCGAAGGTGCAAACTGGAAAGCCTggcgg < 1:3549244/69‑1 (MQ=255)
gAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTggcgg < 1:2194595/69‑1 (MQ=255)
aaCGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCAc > 1:3897707/1‑71 (MQ=255)
aCGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACt < 1:2690449/71‑1 (MQ=255)
tCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGtt < 1:3913105/70‑1 (MQ=255)
tCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGtt > 1:1866286/1‑70 (MQ=255)
ttGATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGc < 1:1906019/71‑1 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg < 1:4528767/70‑1 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg < 1:1670098/70‑1 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg < 1:3616655/70‑1 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:3979501/1‑71 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:23203/1‑71 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:377846/1‑71 (MQ=255)
gATGCCAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGa > 1:1711228/1‑71 (MQ=255)
ccAACGTCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGc > 1:3259306/1‑71 (MQ=255)
tCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg < 1:4570270/60‑1 (MQ=255)
tCAGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCg < 1:4564895/60‑1 (MQ=255)
aGTATGAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt > 1:3922087/1‑71 (MQ=255)
aGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt > 1:4117896/1‑71 (MQ=255)
aGTATCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGAtt > 1:2368298/1‑71 (MQ=255)
aTCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCa > 1:2412330/1‑40 (MQ=37)
aTCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:3477091/1‑71 (MQ=255)
tCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:3324629/1‑70 (MQ=255)
tCAGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:3304571/1‑70 (MQ=255)
aGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:2557560/1‑68 (MQ=255)
aGTGGCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCAc > 1:2305389/1‑68 (MQ=255)
ggCGCGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCag > 1:4099559/1‑68 (MQ=255)
gcgcGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCagaag < 1:3239047/70‑1 (MQ=255)
gcgcGAAGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCagaa > 1:3527691/1‑69 (MQ=255)
aaGGTGCAAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCg > 1:3707677/1‑70 (MQ=255)
|
GAAAAAAGGTAGCCGTTTCTGGAACGTTTCCGGCGTTGATGCCAACGTCAGTATCAGTGGCGCGAAGGTG‑AAACTGGAAAGCCTGGCGGCACTGGTTAACGGTGCGATTGCCTTCGATTCACCAGAAGAGTCG > W3110S.gb/1919868‑1920000
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
