Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	354	41.7	3110465	93.1%	2895842	61.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,927,326	G→A	V148M (GTG→ATG)	sdaC →	predicted serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,927,326	0	G	A	100.0%	221.6 / NA	64	V148M (GTG→ATG)	sdaC	predicted serine transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/51); total (13/51)

ATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATT  >  W3110S.gb/2927284‑2927385

aTGACGCCACCTCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:133823/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1806879/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2368079/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2232373/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2913622/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:887679/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1364167/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:2019410/65‑1 (MQ=255)

aTGACGCCACCGCCGCGTGCGATTCTGGCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:1434732/65‑1 (MQ=255)

    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                                    >  1:690461/1‑64 (MQ=255)

    cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCtt                                    >  1:555594/1‑64 (MQ=255)

     gCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg                                       <  1:658680/60‑1 (MQ=255)

               cgTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                  >  1:779613/1‑39 (MQ=255)

               cgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                        >  1:1479825/1‑65 (MQ=255)

               cgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                        >  1:2059510/1‑65 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1925084/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2960305/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2562387/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2939182/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1980407/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:24580/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:2972701/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:304722/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1868373/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1755703/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:401451/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:527656/54‑1 (MQ=255)

                   cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:939154/54‑1 (MQ=255)

                    gATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                               <  1:1034053/53‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCCGATGATCGTTAAAGCGa          <  1:234083/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:899370/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1002454/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:2882559/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:2601096/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:978375/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1539371/65‑1 (MQ=255)

                             gCTGATCCTGATCATGGGTATGACGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:591677/65‑1 (MQ=255)

                              ctgatcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa          <  1:1870055/64‑1 (MQ=255)

                                 atgctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:396981/4‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:12387/1‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:289257/1‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1292758/1‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1971752/1‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:819489/1‑65 (MQ=255)

                                 atcctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1561018/1‑65 (MQ=255)

                                 atcctgatcATAGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg      >  1:1467884/1‑65 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGtttt  <  1:6419/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1459749/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1381088/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:80564/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:653382/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1292693/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2488006/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1087563/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:536600/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:466883/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:40333/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1911946/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1955509/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2934717/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:199945/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2487858/64‑1 (MQ=255)

                                      gatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:2694027/64‑1 (MQ=255)

                                       atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt  <  1:1670499/63‑1 (MQ=255)

ATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATT  >  W3110S.gb/2927284‑2927385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A