Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F17 I0 R2	276	45.3	3729697	93.0%	3468618	60.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,927,326	G→A	26.4%	V148M (GTG→ATG)	sdaC →	predicted serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,927,326	0	G	A	26.4%	155.8 / 59.9	87	V148M (GTG→ATG)	sdaC	predicted serine transporter
Reads supporting (aligned to +/- strand): ref base G (25/39); new base A (11/12); total (36/51)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

CTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATT  >  W3110S.gb/2927262‑2927393

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg                                                                 >  1:2018488/1‑69 (MQ=255)

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg                                                                 >  1:2014989/1‑69 (MQ=255)

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg                                                                 >  1:381560/1‑69 (MQ=255)

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg                                                                 >  1:1559489/1‑69 (MQ=255)

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTggg                                                                 >  1:3109645/1‑69 (MQ=255)

cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTCATCCTGATCGTggg                                                                 >  1:1106332/1‑69 (MQ=255)

   cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                              >  1:788933/1‑69 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg                                                             <  1:2254021/68‑1 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg                                                             <  1:2897081/68‑1 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg                                                             >  1:1539220/1‑68 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg                                                             <  1:1265966/68‑1 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg                                                             <  1:87789/68‑1 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTCGGTatg                                                             <  1:5739/68‑1 (MQ=255)

     tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTat                                                              <  1:2956988/67‑1 (MQ=255)

       tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                          >  1:3039604/1‑69 (MQ=255)

       tctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                          >  1:377103/1‑69 (MQ=255)

        ctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                          >  1:3611298/1‑68 (MQ=255)

        ctcACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg                                                          >  1:2323389/1‑68 (MQ=255)

                     tATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCt                                             <  1:2606315/68‑1 (MQ=255)

                     tATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCt                                             <  1:2475581/68‑1 (MQ=255)

                     tATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCt                                             <  1:2370775/68‑1 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatgat                                                           >  1:2324595/1‑50 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGCCCATCGTTCGCTTCg                                          >  1:1539912/1‑67 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCAt                                                     <  1:1758599/56‑1 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCg                                          >  1:914710/1‑67 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGt                                        <  1:2562784/69‑1 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGt                                        <  1:1993035/69‑1 (MQ=255)

                         aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt                                        <  1:2199136/69‑1 (MQ=255)

                            ccACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTg                                       >  1:2753466/1‑67 (MQ=255)

                              aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                                       >  1:2619559/1‑65 (MQ=255)

                              aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                                       >  1:316896/1‑65 (MQ=255)

                              aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                                       >  1:3698607/1‑65 (MQ=255)

                              aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTg                                       >  1:2440072/1‑65 (MQ=255)

                                 gccgcGTGTGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:1593854/66‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGCTCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                                >  1:1958683/1‑69 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTTGGTat                                                              <  1:57693/39‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTat                                                              <  1:535932/39‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt                                                 >  1:358410/1‑52 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:2979069/66‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   >  1:2476065/1‑66 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   >  1:2027744/1‑66 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:1539010/66‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:85520/66‑1 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgat                                >  1:3430273/1‑69 (MQ=255)

                                 gccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGagaaga                                 >  1:743956/1‑68 (MQ=255)

                                   cgcgTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatga                              <  1:1826265/69‑1 (MQ=255)

                                       tGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCg                           >  1:3218517/1‑68 (MQ=255)

                                         cGATTCTGTCGCTGATCCTGATCGTGGGTatgatg                                                          <  1:1189738/35‑1 (MQ=255)

                                         cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:2480507/59‑1 (MQ=255)

                                         cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:2787267/59‑1 (MQ=255)

                                         cGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:746485/59‑1 (MQ=255)

                                             tCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc                    <  1:2071036/69‑1 (MQ=255)

                                             tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGc                                              <  1:2398714/43‑1 (MQ=255)

                                             tCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGc                                              <  1:317536/43‑1 (MQ=255)

                                              cTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg                   >  1:3518566/1‑69 (MQ=255)

                                              cTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg                   >  1:675660/1‑69 (MQ=255)

                                              cTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTAGCTTCGGTGAGCAGATGATCGTTAAAGCg                   >  1:2825044/1‑69 (MQ=255)

                                                   gCTGATCCTTATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                        <  1:314433/59‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                        <  1:2336313/59‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                        <  1:3063881/59‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                        <  1:217278/59‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTa                        <  1:320306/59‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:409789/69‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:457659/69‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:1603059/69‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:3325815/69‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:2952403/69‑1 (MQ=255)

                                                   gCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:2473933/69‑1 (MQ=255)

                                                   gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:3047524/69‑1 (MQ=255)

                                                   gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              <  1:486811/69‑1 (MQ=255)

                                                     tgatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa            <  1:1848867/69‑1 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:1557283/45‑1 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:2372465/45‑1 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:2309929/45‑1 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa                                   <  1:225199/45‑1 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAt           >  1:1055174/1‑69 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAt           >  1:3420445/1‑69 (MQ=255)

                                                      gatcctgatcGTGGGTATGATGACCATCGTACGCTTCGGTGAGCa                                   <  1:188510/45‑1 (MQ=255)

                                                         cctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCt        <  1:1385624/69‑1 (MQ=255)

                                                         cctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGAAGATGATCGTTAAAGCGATGAGTATTCt        <  1:2086247/69‑1 (MQ=255)

                                                         cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:1247904/43‑1 (MQ=255)

                                                         cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:3082926/43‑1 (MQ=255)

                                                         cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg                                  <  1:2120966/43‑1 (MQ=255)

                                                          ctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              >  1:1501156/1‑62 (MQ=255)

                                                          ctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg              >  1:1162953/1‑62 (MQ=255)

                                                               cGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTAtt  >  1:1270005/1‑69 (MQ=255)

                                                               cGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTAtt  >  1:779869/1‑69 (MQ=255)

CTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATT  >  W3110S.gb/2927262‑2927393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A