Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,232,470 |
A→G |
L111L (TTG→CTG) |
ygjM ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,232,470 | 0 | A | G | 100.0%
| 126.3
/ NA
| 35 | L111L (TTG→CTG) | ygjM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/3); total (32/3) |
ATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATA > W3110S.gb/3232409‑3232530
|
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:2377135/1‑65 (MQ=255)
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:2343729/1‑65 (MQ=255)
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:2373173/1‑65 (MQ=255)
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:1566937/1‑65 (MQ=255)
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:1449940/1‑65 (MQ=255)
aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc > 1:1357374/1‑65 (MQ=255)
aTGCCGAATCGCGTAGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCagaa > 1:1728748/1‑64 (MQ=255)
gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTTACACCa < 1:774623/61‑1 (MQ=255)
ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCg < 1:1848375/65‑1 (MQ=255)
ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCg < 1:2979156/65‑1 (MQ=255)
cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:949747/1‑58 (MQ=255)
cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:851725/1‑58 (MQ=255)
cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1776746/1‑58 (MQ=255)
cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:3065489/1‑58 (MQ=255)
cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1262034/1‑58 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt > 1:2183362/1‑52 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:999300/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:2467953/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:3097655/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:526474/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:694138/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1169975/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:2372726/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:2043640/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1751060/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1727879/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1694545/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1555647/1‑55 (MQ=255)
tAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:1519736/1‑55 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:2460915/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:2620267/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:2817400/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:2074504/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:490308/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:722175/1‑65 (MQ=255)
cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGCGTTAAACCata > 1:1532896/1‑65 (MQ=255)
|
ATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATA > W3110S.gb/3232409‑3232530
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
