Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R2	328	63.0	4687567	98.0%	4593815	65.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,232,470	A→G	L111L (TTG→CTG)	ygjM ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,232,470	0	A	G	100.0%	192.7 / NA	56	L111L (TTG→CTG)	ygjM	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/32); total (24/32)

GGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC  >  W3110S.gb/3232403‑3232536

ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAc                                                                 >  1:1377908/1‑71 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:993773/71‑1 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:2412766/71‑1 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:4521995/71‑1 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:1399660/71‑1 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:2911642/71‑1 (MQ=255)

    aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt                                                             <  1:1540290/71‑1 (MQ=255)

      aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:209556/1‑71 (MQ=255)

      aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:2577762/1‑71 (MQ=255)

      aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:1646006/1‑71 (MQ=255)

      aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:2917344/1‑71 (MQ=255)

      aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:4431565/1‑71 (MQ=255)

         ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac                                                        >  1:1399971/1‑71 (MQ=255)

             aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATa                                                    <  1:80882/71‑1 (MQ=255)

                gcgTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa                                                           >  1:3765850/1‑61 (MQ=255)

                gcgTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAt                                                 >  1:559647/1‑71 (MQ=255)

                  gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAttt                                               <  1:1145373/71‑1 (MQ=255)

                  gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:4307293/70‑1 (MQ=255)

                     gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACt                                            >  1:2021137/1‑71 (MQ=255)

                     gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACt                                            >  1:1517777/1‑71 (MQ=255)

                          ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac                                                        <  1:1585952/54‑1 (MQ=255)

                          ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGcc                                         <  1:4235665/69‑1 (MQ=255)

                          ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGcc                                         <  1:1454028/69‑1 (MQ=255)

                          ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCaa                                       <  1:2472080/71‑1 (MQ=255)

                            ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt                                     >  1:761702/1‑71 (MQ=255)

                            ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACAATAGATTTACTGCCAAtt                                     >  1:2284223/1‑71 (MQ=255)

                                    tgtTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg                             <  1:2184592/71‑1 (MQ=255)

                                    agtTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg                             <  1:2664521/70‑1 (MQ=255)

                                      ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:2167143/1‑66 (MQ=255)

                                      ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:3542080/1‑66 (MQ=255)

                                          aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:4090176/71‑1 (MQ=255)

                                          aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1163737/71‑1 (MQ=255)

                                          aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1689296/71‑1 (MQ=255)

                                           gCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:1268004/70‑1 (MQ=255)

                                           gCGTTAATTTCCTCTTCCCGCTAAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa                       <  1:2000892/70‑1 (MQ=255)

                                             gTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg                                >  1:2870386/1‑59 (MQ=255)

                                              ttAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGa                                                  <  1:2335178/40‑1 (MQ=255)

                                                aaTTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGt                 >  1:925239/1‑71 (MQ=255)

                                                  tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:765797/38‑1 (MQ=255)

                                                  tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt                                                <  1:1026849/38‑1 (MQ=255)

                                                  tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa               <  1:1497693/71‑1 (MQ=255)

                                                  tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa               <  1:3147462/71‑1 (MQ=255)

                                                    tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa             >  1:4678329/1‑71 (MQ=255)

                                                      ctctTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAAc            >  1:3992029/1‑70 (MQ=255)

                                                        ctTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa          >  1:2818012/1‑70 (MQ=255)

                                                         ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:4049925/59‑1 (MQ=255)

                                                         ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:3612136/59‑1 (MQ=255)

                                                         ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:614959/59‑1 (MQ=255)

                                                         ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg                    <  1:3171642/59‑1 (MQ=255)

                                                         ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa          <  1:4541893/69‑1 (MQ=255)

                                                           cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:666865/1‑71 (MQ=255)

                                                           cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:801880/1‑71 (MQ=255)

                                                           cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:3098754/1‑71 (MQ=255)

                                                           cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt      >  1:2260382/1‑71 (MQ=255)

                                                               cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc  <  1:1177745/71‑1 (MQ=255)

                                                               cTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc  <  1:654921/71‑1 (MQ=255)

GGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC  >  W3110S.gb/3232403‑3232536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A