Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,359,804 |
+A |
coding (752/1419 nt) |
gltD → |
glutamate synthase, 4Fe‑4S protein, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,359,800 | 1 | . | A | 100.0%
| 108.1
/ NA
| 35 | E250K (GAA→AAA) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (35/0); total (35/0) |
TGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAAC > W3110S.gb/3359738‑3359853
|
tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a > 1:1120614/1‑64 (MQ=255)
tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a > 1:247394/1‑64 (MQ=255)
tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a > 1:1011501/1‑64 (MQ=255)
tgAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑a > 1:1887626/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGAc > 1:2581997/1‑63 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:912464/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:2473987/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:239403/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:2345795/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:2258632/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:3081993/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:596314/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:2004781/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1915618/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1882452/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1795105/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:633862/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1351064/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1294650/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:1290767/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACg > 1:635963/1‑64 (MQ=255)
aTGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGC‑GGCTGG‑aa > 1:2975891/1‑52 (MQ=255)
gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc > 1:1859243/1‑65 (MQ=255)
gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc > 1:628751/1‑65 (MQ=255)
gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc > 1:2371626/1‑65 (MQ=255)
gTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc > 1:1266785/1‑65 (MQ=255)
gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc > 1:2264460/1‑65 (MQ=255)
gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc > 1:2861830/1‑65 (MQ=255)
gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc > 1:2852426/1‑65 (MQ=255)
gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc > 1:2196748/1‑65 (MQ=255)
gcgcGGCGGGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGcc > 1:1054047/1‑65 (MQ=255)
cggtggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:2862863/1‑65 (MQ=38)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTc > 1:2848892/1‑56 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCaa > 1:1670944/1‑64 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:2692533/1‑65 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:2183566/1‑65 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:442160/1‑65 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:2175579/1‑65 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:1812244/1‑65 (MQ=255)
cggcggGCTGGAAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAac > 1:1143153/1‑65 (MQ=255)
|
TGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAAC > W3110S.gb/3359738‑3359853
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
