Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,359,804 |
+A |
coding (752/1419 nt) |
gltD → |
glutamate synthase, 4Fe‑4S protein, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,359,800 | 1 | . | A | 100.0%
| 11.7
/ NA
| 5 | E250K (GAA→AAA) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/5); total (0/5) |
TGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGG > W3110S.gb/3359756‑3359819
|
tgtTCCTTGGCGTCGGGACTTCTCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2399819/65‑1 (MQ=255)
tgtTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:1450777/65‑1 (MQ=255)
tgtTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:3254/65‑1 (MQ=255)
ccTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2053182/61‑1 (MQ=255)
aCTTATCAGTCAATGCGCGGCGGGCTGGAAAAACGAAGACGCCGATgg < 1:2000095/48‑1 (MQ=39)
|
TGTTCCTTGGCGTCGGGACTTATCAGTCAATGCGCGGCGGGCTGG‑AAAACGAAGACGCCGATGG > W3110S.gb/3359756‑3359819
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
