Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,224,676 |
T→C |
15.5% |
F180L (TTT→CTT) |
yebU → |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,224,676 | 0 | T | C | 15.5%
| 95.0
/ 12.1
| 45 | F180L (TTT→CTT) | yebU | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base C (4/3); total (24/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTG > minE/1224610‑1224744
|
ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTg < 1:385961/70‑1 (MQ=255)
aCATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTg > 1:946138/1‑71 (MQ=255)
aCATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTg > 1:347036/1‑71 (MQ=255)
cATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGtt < 1:528674/65‑1 (MQ=255)
cATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGc > 1:1322747/1‑71 (MQ=255)
tatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCgtgt < 1:982007/56‑1 (MQ=255)
tatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCgtgt < 1:809789/56‑1 (MQ=255)
tatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGCTTGGTg < 1:282298/62‑1 (MQ=255)
tCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGc > 1:2041091/1‑69 (MQ=255)
aGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGa < 1:374857/71‑1 (MQ=255)
aGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGa < 1:2138620/71‑1 (MQ=255)
gTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGa < 1:78366/70‑1 (MQ=255)
tCAGTAATGTTGCGCTCACACATTTTGATTGCCGCGTGTTTGGTGCGGCAGTGCCAGa < 1:1900726/58‑1 (MQ=255)
tCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGaaa < 1:407742/60‑1 (MQ=255)
tCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGaaa < 1:51281/60‑1 (MQ=255)
tCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGa < 1:176597/58‑1 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt < 1:2134182/71‑1 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt < 1:345797/71‑1 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt > 1:2044485/1‑71 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt < 1:1968567/71‑1 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt > 1:967750/1‑71 (MQ=255)
aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGCTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAt > 1:1935026/1‑71 (MQ=255)
tAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAttt < 1:745175/71‑1 (MQ=255)
aTGTGGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTGCGATGCCATTTTg < 1:312921/71‑1 (MQ=255)
ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTg > 1:1866953/1‑71 (MQ=255)
gcgcTCACACATTTTGATGGCCGCGTGCTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTgg < 1:345264/70‑1 (MQ=255)
cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCAtttt > 1:1834391/1‑60 (MQ=255)
cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGa > 1:313797/1‑66 (MQ=255)
cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGa > 1:1596242/1‑66 (MQ=255)
cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGAcaga > 1:551359/1‑67 (MQ=255)
cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTc > 1:1029651/1‑71 (MQ=255)
acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTcc < 1:2041844/71‑1 (MQ=255)
acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTcc > 1:471022/1‑71 (MQ=255)
acaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTccc > 1:103255/1‑70 (MQ=255)
caTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGc < 1:2043265/58‑1 (MQ=255)
caTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTg > 1:600626/1‑71 (MQ=255)
aTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTc < 1:1411689/66‑1 (MQ=255)
aTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTc < 1:1368490/66‑1 (MQ=255)
tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctc > 1:618336/1‑71 (MQ=255)
tttGATGGCCGCGTGCTTGGTGCTGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctc > 1:86396/1‑71 (MQ=255)
ttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctct > 1:1716045/1‑71 (MQ=255)
ttGATGGCCGCGTGCTTGGTGCGGCAGTGCCAGAAATGTTCGa > 1:1129188/1‑43 (MQ=255)
ttGATGGCCGCGTGCTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctct > 1:542177/1‑71 (MQ=255)
ttGATGGCCGCGTGCTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctc < 1:1662571/70‑1 (MQ=255)
gATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTgg > 1:479005/1‑71 (MQ=255)
aTGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGc > 1:1805690/1‑71 (MQ=255)
gcgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGctc > 1:237444/1‑61 (MQ=255)
gcgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGc < 1:251832/71‑1 (MQ=255)
gtgtTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgt < 1:688542/71‑1 (MQ=255)
tgtTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtg > 1:1524047/1‑71 (MQ=255)
tttGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCt > 1:355050/1‑52 (MQ=255)
|
TTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTG > minE/1224610‑1224744
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
