Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,732,642 |
C→A |
26.6% |
Q238K (CAA→AAA) |
yheO → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,732,642 | 0 | C | A | 26.6%
| 195.6
/ 34.8
| 75 | Q238K (CAA→AAA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (29/26); new base A (7/13); total (36/39) |
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGT > minE/2732573‑2732706
|
cGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:885262/71‑1 (MQ=255)
cGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTGCAAGAGCGGTGATTTCCAGGGGCa < 1:42554/71‑1 (MQ=255)
gCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGc > 1:1922383/1‑69 (MQ=255)
gCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGc > 1:1830224/1‑69 (MQ=255)
cTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa < 1:767084/71‑1 (MQ=255)
tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCTGTGATTTCCAGGGGAAAGa > 1:695469/1‑70 (MQ=255)
tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa > 1:985663/1‑70 (MQ=255)
tGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGa > 1:1966758/1‑70 (MQ=255)
aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCCAGAt < 1:1243271/69‑1 (MQ=255)
aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:405311/69‑1 (MQ=255)
aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt > 1:407284/1‑69 (MQ=255)
aaCATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:1977724/69‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2121814/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:1422886/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:684331/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGt < 1:2202362/71‑1 (MQ=255)
aaaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGt < 1:1895095/71‑1 (MQ=255)
aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:1070009/71‑1 (MQ=255)
aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:739799/71‑1 (MQ=255)
aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGAGCa < 1:922092/55‑1 (MQ=255)
acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTg > 1:1051446/1‑71 (MQ=255)
acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTg > 1:1505490/1‑71 (MQ=255)
tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:1067045/1‑55 (MQ=255)
tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:1241433/1‑55 (MQ=255)
tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTa > 1:2035867/1‑55 (MQ=255)
cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:908589/1‑55 (MQ=255)
cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2109677/1‑55 (MQ=255)
tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt > 1:1008102/1‑57 (MQ=255)
tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:1415204/1‑69 (MQ=255)
ctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:1727038/1‑45 (MQ=255)
ctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAc > 1:1976588/1‑71 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggtg < 1:1823605/68‑1 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:454894/64‑1 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:2157085/69‑1 (MQ=255)
aCATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt > 1:1963611/1‑54 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1189174/1‑46 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1452759/1‑46 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1623304/1‑46 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:1191766/1‑46 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTaa > 1:636851/1‑46 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:1470870/1‑71 (MQ=255)
tCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:55372/49‑1 (MQ=255)
tCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:1253548/49‑1 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:625557/1‑52 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:395258/1‑52 (MQ=255)
gCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1414322/71‑1 (MQ=255)
cccAGTTCAAGAGCGGTGATTTCCAGGGGCAAGAt < 1:1430882/34‑1 (MQ=255)
aGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:1526928/1‑70 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtgg > 1:1867743/1‑52 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGAATACgg > 1:1376663/1‑71 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACg < 1:550887/70‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGt < 1:165313/38‑1 (MQ=255)
tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1761943/55‑1 (MQ=255)
tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:2148800/55‑1 (MQ=255)
tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:1148417/55‑1 (MQ=255)
tGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACa < 1:1721720/71‑1 (MQ=255)
gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:399966/1‑45 (MQ=255)
gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:1923024/1‑45 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:2047951/39‑1 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:2045332/39‑1 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:1613640/39‑1 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:1422635/1‑71 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTAACCGGGCCAGCATACGGTACGCAACAgg > 1:1139765/1‑71 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATAGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:1076544/1‑71 (MQ=255)
tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:579357/43‑1 (MQ=255)
tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:1396288/43‑1 (MQ=255)
tttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa < 1:893889/43‑1 (MQ=255)
tttCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtg < 1:290496/39‑1 (MQ=255)
tttCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgtg < 1:189138/39‑1 (MQ=255)
ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGGATACGGTACGCAACAGGc < 1:1314628/70‑1 (MQ=255)
ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc < 1:416223/70‑1 (MQ=255)
tCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAcc > 1:1198030/1‑43 (MQ=255)
cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:427119/1‑50 (MQ=255)
cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa > 1:1210211/1‑50 (MQ=255)
cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagt < 1:750703/71‑1 (MQ=255)
cAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagt < 1:1586766/71‑1 (MQ=255)
ggCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACg > 1:2026762/1‑50 (MQ=255)
|
CGCCTGAACATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGT > minE/2732573‑2732706
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
