Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,732,642 |
C→A |
25.2% |
Q238K (CAA→AAA) |
yheO → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,732,642 | 0 | C | A | 25.2%
| 309.3
/ 66.8
| 123 | Q238K (CAA→AAA) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (49/43); new base A (6/25); total (55/68) |
| Fisher's exact test for biased strand distribution p-value = 1.48e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 |
Only 100 of 123 total aligned reads displayed.
CATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTA > minE/2732581‑2732707
|
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2981059/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:3311196/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2032671/71‑1 (MQ=255)
cATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:3649291/71‑1 (MQ=255)
aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAg > 1:565243/1‑69 (MQ=255)
aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt > 1:15885/1‑70 (MQ=255)
aTCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt > 1:737119/1‑70 (MQ=255)
ccAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGc > 1:1622950/1‑71 (MQ=255)
cAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCg > 1:1032502/1‑71 (MQ=255)
cAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCg > 1:1575593/1‑71 (MQ=255)
aaCACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGtt < 1:4013334/71‑1 (MQ=255)
acacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt < 1:3374529/71‑1 (MQ=255)
acacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGttt < 1:269994/71‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:246867/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:2134761/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:3665366/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:2673847/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:3018267/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:1029325/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:1090735/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAg < 1:133019/55‑1 (MQ=255)
cacacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2937503/61‑1 (MQ=255)
acacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:490406/60‑1 (MQ=255)
cacTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2569979/1‑61 (MQ=255)
acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGt < 1:2424768/58‑1 (MQ=255)
acTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGcc < 1:871681/71‑1 (MQ=255)
tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCg > 1:1539235/1‑71 (MQ=255)
tCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCg > 1:3462550/1‑71 (MQ=255)
cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:2685286/1‑55 (MQ=255)
cTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTaa > 1:3554560/1‑55 (MQ=255)
cTATCTCTACATACGCCAGTTCAAGAGAGGTGATTTCAAGGGGCAAg > 1:131325/1‑47 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:2647344/42‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:331444/42‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCa < 1:1492347/42‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:2337703/1‑71 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgtggt > 1:634627/1‑71 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:1386101/68‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:925224/68‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:2513328/68‑1 (MQ=255)
tctcTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:3661756/68‑1 (MQ=255)
tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:3786448/1‑71 (MQ=255)
tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:3771394/1‑71 (MQ=255)
tctACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGa > 1:1883879/1‑71 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:573842/64‑1 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:578603/64‑1 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCgt < 1:4019528/64‑1 (MQ=255)
tACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGAcc > 1:1751125/1‑71 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:82993/1‑56 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:2894421/1‑56 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGc > 1:1476020/1‑56 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:234318/1‑71 (MQ=255)
cATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg > 1:3200817/1‑71 (MQ=255)
cATCCGCCAGTTAAAGAGCGGTGATTTCCAGGGGCAAg > 1:317090/1‑38 (MQ=255)
ccGCAAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATATGTAATGCTTTTTGCCATCgt < 1:2027913/59‑1 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:3958651/1‑38 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:4076715/1‑38 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:150529/1‑38 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:332245/1‑38 (MQ=255)
cGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATaa > 1:3275630/1‑38 (MQ=255)
gCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAg < 1:3190136/71‑1 (MQ=255)
cAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:2121230/71‑1 (MQ=255)
cAGTTCAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCa < 1:958249/71‑1 (MQ=255)
gTTCAAGAGCGGTGATTTACAGGGGAAAGATAAGTAATGCGTTTTGCCATCgt < 1:1263685/53‑1 (MQ=255)
ttCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCgg < 1:3025540/61‑1 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGcc > 1:3148239/1‑45 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2787227/1‑71 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2256889/1‑71 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:2772987/1‑71 (MQ=255)
cAAGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg > 1:3913832/1‑71 (MQ=255)
aaGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:2578238/70‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:683657/71‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:960870/71‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:965631/71‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2233162/71‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2541079/71‑1 (MQ=255)
aaGAGCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:344930/71‑1 (MQ=255)
agagCGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGt < 1:2865868/70‑1 (MQ=255)
cGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:2397275/71‑1 (MQ=255)
cGGTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:1692960/71‑1 (MQ=255)
ggTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCa < 1:13637/70‑1 (MQ=255)
gTGATTTCCAGGGGCAAGATAAGTATTGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:516603/1‑70 (MQ=255)
gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:1617698/1‑70 (MQ=255)
gTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCaa > 1:1168214/1‑70 (MQ=255)
gTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAAc < 1:3730519/71‑1 (MQ=255)
gTGATTTCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAAc < 1:4082849/71‑1 (MQ=255)
tGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATa < 1:490011/59‑1 (MQ=255)
gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATc > 1:3456275/1‑38 (MQ=255)
gATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:2852991/1‑71 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACgg < 1:835565/60‑1 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAgg > 1:2733862/1‑71 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAg > 1:3094202/1‑70 (MQ=255)
aTTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCACCATACgg < 1:113564/60‑1 (MQ=255)
ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTa > 1:3962326/1‑60 (MQ=255)
ttCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTa > 1:2781763/1‑60 (MQ=255)
tCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc > 1:1742456/1‑69 (MQ=255)
tCCAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGc > 1:2904462/1‑69 (MQ=255)
cAGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGag < 1:881378/70‑1 (MQ=255)
aGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATa < 1:1768020/51‑1 (MQ=255)
aGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagta < 1:1928465/71‑1 (MQ=255)
aGGGGAAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGagta < 1:1849283/71‑1 (MQ=255)
|
CATCTCCAAACACACTGTCTATCTCTACATCCGCCAGTTCAAGAGCGGTGATTTCCAGGGGCAAGATAAGTAATGCGTTTTGCCATCGTGGTGACCGGGCCAGCATACGGTACGCAACAGGCGAGTA > minE/2732581‑2732707
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
