Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F17 I0 R2
|
278 |
43.2 |
2215644 |
94.6% |
2095999 |
61.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,912,429 |
C→T |
23.9% |
A135T (GCG→ACG) |
pyrI ← |
aspartate carbamoyltransferase, regulatory subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,912,429 | 0 | C | T | 23.9%
| 91.1
/ 23.8
| 46 | A135T (GCG→ACG) | pyrI | aspartate carbamoyltransferase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base T (6/5); total (19/27) |
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTG > minE/2912363‑2912499
|
aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:261017/1‑69 (MQ=255)
cGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:738073/1‑70 (MQ=255)
cAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt < 1:135103/71‑1 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgcg > 1:432723/1‑71 (MQ=255)
ttGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc < 1:754081/68‑1 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:718728/1‑58 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat > 1:4802/1‑58 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt < 1:1744947/71‑1 (MQ=255)
aGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCg > 1:1792864/1‑71 (MQ=255)
aGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCCTTTTCg < 1:284599/71‑1 (MQ=255)
gCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGa < 1:1260931/51‑1 (MQ=255)
caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCa > 1:606914/1‑71 (MQ=255)
caccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa < 1:894435/50‑1 (MQ=255)
accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCAc > 1:8927/1‑71 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:321195/1‑70 (MQ=255)
caTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCTCGATATc < 1:1607534/49‑1 (MQ=255)
caTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGc > 1:1862747/1‑70 (MQ=255)
ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat < 1:2037611/46‑1 (MQ=255)
ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:564482/47‑1 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCa > 1:1546264/1‑47 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaac > 1:433307/1‑70 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAg > 1:563178/1‑71 (MQ=255)
aTGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGc > 1:1460679/1‑71 (MQ=255)
tGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGtttt > 1:1719454/1‑57 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1947752/42‑1 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1695175/42‑1 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:448748/42‑1 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTgg < 1:673316/71‑1 (MQ=255)
aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGCCAAAGCTGGATg < 1:593037/71‑1 (MQ=255)
aaCTCTTTTTCACAGTATTTGCATTTGAGAGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:2046234/71‑1 (MQ=255)
aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaa < 1:1964004/71‑1 (MQ=255)
aCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGa < 1:1271266/70‑1 (MQ=255)
ctctTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaaa > 1:906837/1‑71 (MQ=255)
tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg > 1:1880122/1‑71 (MQ=255)
tttttCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:39912/36‑1 (MQ=255)
tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc < 1:759058/68‑1 (MQ=255)
tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg < 1:1164606/69‑1 (MQ=255)
tCACAGTATTTGCATTTGAGCGCGATATCATTTGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt < 1:1122150/70‑1 (MQ=255)
acaGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg < 1:88153/71‑1 (MQ=255)
tATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTg < 1:1616031/51‑1 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCAt < 1:714266/68‑1 (MQ=255)
tGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg > 1:1616690/1‑67 (MQ=255)
cATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGa < 1:288496/70‑1 (MQ=255)
aTTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa < 1:322211/71‑1 (MQ=255)
aTTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGATGATa < 1:1742333/71‑1 (MQ=255)
ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:367367/1‑69 (MQ=255)
cgATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTg > 1:2141700/1‑71 (MQ=255)
|
ACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAGTTGCTG > minE/2912363‑2912499
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
