Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F3 I0 R2
|
232 |
76.3 |
4135488 |
90.9% |
3759158 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
2,912,429 |
C→T |
35.9% |
A135T (GCG→ACG) |
pyrI ← |
aspartate carbamoyltransferase, regulatory subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 2,912,429 | 0 | C | T | 35.9%
| 97.1
/ 92.3
| 92 | A135T (GCG→ACG) | pyrI | aspartate carbamoyltransferase, regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base C (34/25); new base T (23/10); total (57/35) |
| Fisher's exact test for biased strand distribution p-value = 2.73e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 101 total aligned reads displayed.
CCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAG > minE/2912360‑2912493
|
ccAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:2064041/70‑1 (MQ=255)
aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:964516/1‑71 (MQ=255)
aCCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:3590656/1‑71 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACATTATTTGCATTTGAGCGCGatat < 1:1260040/65‑1 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:1616209/68‑1 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:2290086/68‑1 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:2191697/68‑1 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1139186/66‑1 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:329704/1‑71 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:324886/1‑71 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:2904254/1‑71 (MQ=255)
ttaattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg > 1:1385635/1‑71 (MQ=255)
taattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGgc > 1:4117360/1‑71 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:522943/1‑68 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:2707443/1‑68 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTg > 1:2551459/1‑68 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt > 1:2678439/1‑66 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAt > 1:1157822/1‑66 (MQ=255)
aattGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTAGCATTTGAGCGTGATATCATTg > 1:1061596/1‑68 (MQ=255)
ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCTATATCATTGgcgc > 1:2017634/1‑68 (MQ=255)
ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2437108/1‑71 (MQ=255)
ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2879982/1‑71 (MQ=255)
ggCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtt > 1:2445943/1‑71 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:3152953/57‑1 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:3675213/57‑1 (MQ=255)
gCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGata < 1:632041/57‑1 (MQ=255)
cAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCAt < 1:854705/59‑1 (MQ=255)
gCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg > 1:1304614/1‑70 (MQ=255)
accacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATc < 1:1988123/53‑1 (MQ=255)
ccacATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:1808550/46‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:3590284/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:383145/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:2709613/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:400074/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:3322964/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:1368700/45‑1 (MQ=255)
cacaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgcgc < 1:970115/45‑1 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGc < 1:138714/71‑1 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:784327/1‑70 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:2588901/1‑70 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1129937/1‑70 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1138894/1‑70 (MQ=255)
acaTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg > 1:1931202/1‑70 (MQ=255)
attattGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGatat < 1:1529221/47‑1 (MQ=255)
aTTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGa < 1:645232/44‑1 (MQ=255)
ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:1642185/50‑1 (MQ=255)
ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:1778403/50‑1 (MQ=255)
ttATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt < 1:308301/50‑1 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:8942/1‑44 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:3990477/1‑44 (MQ=255)
tATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGata > 1:1831537/1‑44 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa > 1:3623444/1‑43 (MQ=255)
ggAAAACTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCa > 1:3257843/1‑43 (MQ=255)
gAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:3705553/1‑37 (MQ=255)
gAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGa > 1:287165/1‑37 (MQ=255)
aaaCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt > 1:2347275/1‑52 (MQ=255)
aaCTCTTTTTCACATTATTTGCATTTGAGCGTGATATc < 1:441861/38‑1 (MQ=38)
aCTCTTTTTCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGaa < 1:3686885/71‑1 (MQ=255)
ctctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGaaa < 1:3308653/71‑1 (MQ=255)
ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGa > 1:2990516/1‑64 (MQ=255)
ctTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGa > 1:2252724/1‑64 (MQ=255)
tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGc > 1:2409409/1‑49 (MQ=255)
tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCg > 1:3317074/1‑71 (MQ=255)
tttttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCg > 1:3508241/1‑71 (MQ=255)
tttCACAGTATTTGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg < 1:3618520/70‑1 (MQ=255)
tttCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg < 1:1191959/70‑1 (MQ=255)
caGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGc < 1:2010960/71‑1 (MQ=255)
tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCgg < 1:1538575/67‑1 (MQ=255)
tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCgg < 1:1408860/67‑1 (MQ=255)
tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:4030871/70‑1 (MQ=255)
tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:1588314/70‑1 (MQ=255)
tATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCAt < 1:3820367/70‑1 (MQ=255)
aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg < 1:872847/37‑1 (MQ=255)
aTTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg < 1:2702091/37‑1 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1472507/1‑71 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1140248/1‑71 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3161124/1‑71 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1872261/1‑71 (MQ=255)
tttGCATTTGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:3451941/1‑71 (MQ=255)
tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:1362155/1‑71 (MQ=255)
tttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGc > 1:2331646/1‑71 (MQ=255)
ttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa > 1:3048840/1‑37 (MQ=255)
ttGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa > 1:3354939/1‑37 (MQ=255)
tGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCt < 1:1212955/70‑1 (MQ=255)
tGCATTTGAGCGCGATATCATTGGCGCCTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCt < 1:3864400/70‑1 (MQ=255)
tttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGc < 1:2263400/41‑1 (MQ=255)
tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTg < 1:3900055/43‑1 (MQ=255)
tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATAc < 1:2954653/71‑1 (MQ=255)
tttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATAc < 1:1523787/71‑1 (MQ=255)
ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:492105/1‑69 (MQ=255)
ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:3705256/1‑69 (MQ=255)
ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:71025/1‑69 (MQ=255)
ttGAGCGTGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:3491964/1‑69 (MQ=255)
ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAAGCTGGATGAAACCGGTTCGGCATg > 1:1706521/1‑63 (MQ=255)
ttGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATa > 1:2189622/1‑69 (MQ=255)
tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:801604/1‑52 (MQ=255)
tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:2096147/1‑52 (MQ=255)
tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:3580398/1‑52 (MQ=255)
tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCgg > 1:3886516/1‑52 (MQ=255)
tGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAg > 1:1338453/1‑71 (MQ=255)
|
CCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGC‑AAAGCTGGATGAAACCGGTTCGGCATGGCTGATACAG > minE/2912360‑2912493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
