Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F28 I1 R2	146	74.7	3299319	84.8%	2797822	66.9

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,681,918	A→G	R598R (CGT→CGC)	yhgF ←	predicted transcriptional accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,681,918	0	A	G	100.0%	109.2 / NA	30	R598R (CGT→CGC)	yhgF	predicted transcriptional accessory protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (5/25); total (5/25)

GATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCAT > minE/2681855‑2681941

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:2539147/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:401180/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:3212725/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:3059259/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:708390/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:2608888/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1240310/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1335545/70‑1 (MQ=255)

gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1608780/70‑1 (MQ=255)

 atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1089061/69‑1 (MQ=255)

 atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:98449/69‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1577833/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:334376/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:3082918/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:3042400/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:2978857/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:653786/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:2644519/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:722155/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1940146/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1835027/71‑1 (MQ=255)

                gTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:1767083/71‑1 (MQ=255)

                 tCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:819154/70‑1 (MQ=255)

                     cacaCCGAATTTCTCATGAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:1132922/1‑66 (MQ=255)

                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2917593/1‑66 (MQ=255)

                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2653611/1‑66 (MQ=255)

                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:2327544/1‑66 (MQ=255)

                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  >  1:726032/1‑66 (MQ=255)

                          cGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt  <  1:172820/61‑1 (MQ=255)

                             aTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGt                   <  1:1953987/41‑1 (MQ=255)

GATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCAT > minE/2681855‑2681941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A