Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R2
|
422 |
46.8 |
3726540 |
94.7% |
3529033 |
61.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,887,874 |
T→C |
100% |
F106L (TTT→CTT) |
yeaX → |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,887,874 | 0 | T | C | 86.3%
| 140.3
/ 11.4
| 51 | F106L (TTT→CTT) | yeaX | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (7/0); new base C (31/13); total (38/13) |
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCT > W3110S.gb/1887808‑1887936
|
tCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTgc > 1:3015153/1‑71 (MQ=255)
cGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:1783868/71‑1 (MQ=255)
ttttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAtt > 1:122848/1‑71 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc > 1:2678780/1‑70 (MQ=255)
ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:104994/54‑1 (MQ=255)
ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:2100294/54‑1 (MQ=255)
ggTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:420829/54‑1 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:1750350/1‑71 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:3179295/1‑71 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:1062321/1‑71 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAg > 1:2382214/1‑71 (MQ=255)
aaaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:1443539/1‑53 (MQ=255)
aaaaGTGGGCGATCGGTTAACGATTTCAAAGCCTAATAACCTGCTTGCGCTaa > 1:2691187/1‑53 (MQ=39)
aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCa > 1:3229783/1‑60 (MQ=255)
aaaGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCa > 1:3109880/1‑71 (MQ=255)
cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa > 1:3241105/1‑71 (MQ=255)
cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa > 1:1309662/1‑71 (MQ=255)
cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:1319085/1‑40 (MQ=255)
cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:2153165/1‑40 (MQ=255)
cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:629015/1‑71 (MQ=255)
cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:3010822/1‑71 (MQ=255)
ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg < 1:2358358/71‑1 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTaa > 1:3554563/1‑35 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:951999/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:1513899/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3672399/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3550059/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:3276910/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:2256406/1‑71 (MQ=255)
aaCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcg > 1:2994346/1‑71 (MQ=255)
aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:784903/71‑1 (MQ=255)
aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:1029054/71‑1 (MQ=255)
aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg < 1:3644861/71‑1 (MQ=255)
aCGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:2143488/71‑1 (MQ=255)
aCGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:3306114/71‑1 (MQ=255)
ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcg > 1:79083/1‑48 (MQ=255)
ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:3655807/1‑71 (MQ=255)
ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:920369/1‑71 (MQ=255)
ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:2840976/1‑71 (MQ=255)
ataaCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCt > 1:3051749/1‑71 (MQ=255)
ataaCCTGTTTGCGCTAATTCCATCAGCCAGAAAGCATc > 1:3364703/1‑39 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:2356240/1‑49 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:818088/1‑49 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:898621/1‑49 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:1693093/1‑49 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:2554326/1‑49 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcg > 1:1469608/1‑48 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTAAGCCAGAAAGCATCTGTTTATcgc > 1:3653869/1‑49 (MQ=255)
taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:2298909/60‑1 (MQ=255)
taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:3526463/60‑1 (MQ=255)
taaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg < 1:515787/60‑1 (MQ=255)
|
TCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCCCT > W3110S.gb/1887808‑1887936
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
