Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R2
|
260 |
44.3 |
3360226 |
90.5% |
3041004 |
64.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,887,874 |
T→C |
68.6% |
F106L (TTT→CTT) |
yeaX → |
predicted oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,887,874 | 0 | T | C | 68.6%
| 63.4
/ 40.6
| 51 | F106L (TTT→CTT) | yeaX | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (14/2); new base C (11/24); total (25/26) |
| Fisher's exact test for biased strand distribution p-value = 2.42e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01 |
CCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCC > W3110S.gb/1887810‑1887934
|
ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTtcgc < 1:505071/71‑1 (MQ=255)
ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTgcgc < 1:2015295/71‑1 (MQ=255)
ccGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTgcgc < 1:1550269/71‑1 (MQ=255)
tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt < 1:2453186/69‑1 (MQ=255)
tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt < 1:2871298/69‑1 (MQ=255)
tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt < 1:486743/69‑1 (MQ=255)
tttGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAAt < 1:832139/69‑1 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc < 1:1159750/70‑1 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc < 1:442610/70‑1 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc < 1:1985682/70‑1 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc < 1:355485/70‑1 (MQ=255)
agcagGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGc < 1:1049419/70‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:971362/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:1167384/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:972942/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:1902258/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:3198472/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:2873704/53‑1 (MQ=255)
gTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:98235/53‑1 (MQ=255)
gTAAAAGTGGGCAATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:907418/53‑1 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:816745/52‑1 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:382507/52‑1 (MQ=255)
tAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCt < 1:2762760/52‑1 (MQ=255)
ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt < 1:1265548/71‑1 (MQ=255)
cGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgc > 1:3052798/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCATAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:3254766/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:3174713/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:710621/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:8045/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:2498686/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:2000090/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:3285196/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:1489456/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:1473543/1‑71 (MQ=255)
ttCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTg > 1:1049416/1‑71 (MQ=255)
ttCAACCCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTTTTTATCGCGGGCGGTATTg > 1:905/1‑71 (MQ=255)
aCGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:1039895/71‑1 (MQ=255)
aCGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTAt < 1:1870212/71‑1 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:1945439/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:879518/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:1243963/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:194538/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:2072966/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:3111262/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:3046155/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:2320563/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAccc > 1:2779430/1‑69 (MQ=255)
ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAcc > 1:2538899/1‑68 (MQ=255)
aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa > 1:1715607/1‑54 (MQ=255)
aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa > 1:2282227/1‑54 (MQ=255)
aCCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTa > 1:1093129/1‑54 (MQ=255)
|
CCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCACCC > W3110S.gb/1887810‑1887934
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
