Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
517,941 |
A→G |
23.8% |
S145P (TCA→CCA) |
ybbO ← |
predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 517,941 | 0 | A | G | 23.8%
| 73.7
/ 22.3
| 42 | S145P (TCA→CCA) | ybbO | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (17/15); new base G (6/4); total (23/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGC > W3110S.gb/517876‑518010
|
cAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGtc < 1:4366666/70‑1 (MQ=255)
cAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtca > 1:4570666/1‑71 (MQ=255)
gcgcATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtcat < 1:4361566/70‑1 (MQ=255)
cATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATcac > 1:3737313/1‑70 (MQ=255)
tatTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGtc < 1:1007515/63‑1 (MQ=255)
gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCtt > 1:645962/1‑70 (MQ=255)
gCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCtt < 1:2269572/70‑1 (MQ=255)
cgcgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCAc > 1:2708911/1‑71 (MQ=255)
cgTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACaa > 1:686707/1‑57 (MQ=255)
tAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTg < 1:90826/71‑1 (MQ=255)
tAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:1867059/1‑55 (MQ=255)
cgcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCgg > 1:2381455/1‑70 (MQ=255)
gcCACGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGt < 1:1415530/70‑1 (MQ=255)
ccACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTc < 1:275148/59‑1 (MQ=255)
ccACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTc < 1:2668411/59‑1 (MQ=255)
cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGccc < 1:4277268/55‑1 (MQ=255)
cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:2360394/1‑70 (MQ=255)
cACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:4193161/1‑70 (MQ=255)
aCGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACa > 1:2834666/1‑71 (MQ=255)
aCGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:563849/1‑47 (MQ=255)
aCGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACaa > 1:630392/1‑47 (MQ=255)
aCGACCCGGCGTGGAGATTAATCCCATAACCGATGATGTCATCACAAt > 1:2465047/1‑48 (MQ=255)
cGACCCGGCGTGGAGATTAATCCCATCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACAt > 1:4070924/1‑71 (MQ=255)
aCCCGGCGTGGAGATTAATCCCATCACAGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATcg > 1:3550791/1‑71 (MQ=255)
tGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:3384101/52‑1 (MQ=255)
tGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGt < 1:4053913/68‑1 (MQ=255)
gagaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACa > 1:1957942/1‑70 (MQ=255)
agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATc < 1:1856960/59‑1 (MQ=255)
agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAgg > 1:2823680/1‑71 (MQ=255)
agaTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAgg > 1:2354435/1‑71 (MQ=255)
aTTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTa < 1:782204/52‑1 (MQ=255)
aTCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATg < 1:3282847/71‑1 (MQ=255)
tCACCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCAt < 1:3791607/64‑1 (MQ=255)
tCACCGATGATGTCATCACAATACGCCCTTCACCg > 1:2298960/1‑35 (MQ=255)
tCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:3313365/37‑1 (MQ=255)
tCACCGATGATGTCATCACAATACGCCCTTCACCGTg < 1:223622/37‑1 (MQ=255)
aCCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTaa > 1:3914417/1‑41 (MQ=255)
aCCGATGGTGTCATCACAATACGCCCTTCACCGTGCGGTAACATcg > 1:2449003/1‑46 (MQ=255)
aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACa > 1:1762941/1‑43 (MQ=255)
aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTaa > 1:1111466/1‑53 (MQ=255)
aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCt < 1:3953326/70‑1 (MQ=255)
aCCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGCTGAGCt < 1:1695718/70‑1 (MQ=255)
gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg < 1:2193452/68‑1 (MQ=255)
gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTg < 1:2193142/68‑1 (MQ=255)
gatgatGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtg < 1:3156235/71‑1 (MQ=255)
atgatgTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGgtgt > 1:2617613/1‑71 (MQ=255)
gatgTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTgc > 1:212829/1‑71 (MQ=255)
|
CAGCGCATATTTACTGGCCGCGTAAGCGCCACGACCCGGCGTGGAGATTAATCCCATCACCGATGATGTCATCACAATACGCCCTTCACCGTGCGGTAACATCGCGGGTAACAGGCGCATGGTGAGCTGGTGTGC > W3110S.gb/517876‑518010
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
