Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
571,256 |
A→G |
21.4% |
T194A (ACA→GCA) |
ybcM → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 571,256 | 0 | A | G | 21.4%
| 141.7
/ 35.7
| 70 | T194A (ACA→GCA) | ybcM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (31/24); new base G (5/10); total (36/34) |
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGCTATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAAATAA > W3110S.gb/571187‑571326
|
ctgctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCta < 1:1430326/70‑1 (MQ=255)
ctgctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCta < 1:3571743/70‑1 (MQ=255)
ctATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgcat < 1:190752/70‑1 (MQ=255)
aTCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:958767/1‑70 (MQ=255)
tCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:2229949/70‑1 (MQ=255)
tCCGAACACGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:2732700/70‑1 (MQ=255)
cGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATc > 1:1458243/1‑71 (MQ=255)
aCCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgca > 1:2341005/1‑61 (MQ=255)
aCCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGcc > 1:1290360/1‑71 (MQ=255)
ggCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCt < 1:2052440/71‑1 (MQ=255)
gCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:2453276/57‑1 (MQ=255)
cacaAAATGGACAGCAAACTAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCg < 1:724799/63‑1 (MQ=255)
acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacattac < 1:4280860/58‑1 (MQ=255)
acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:2892081/55‑1 (MQ=255)
acaAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAg > 1:343371/1‑70 (MQ=255)
caAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATc < 1:1177075/60‑1 (MQ=255)
caAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc > 1:3432677/1‑63 (MQ=255)
aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAg > 1:2371494/1‑71 (MQ=255)
aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTa > 1:317659/1‑66 (MQ=255)
aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCaa > 1:3477542/1‑70 (MQ=255)
aaaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:1412977/1‑71 (MQ=255)
aaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4426142/71‑1 (MQ=255)
aaTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:844771/71‑1 (MQ=255)
aaTGGACAGCAAACAAGGTTGCTCGATATCTATACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc > 1:571871/1‑71 (MQ=255)
aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCg < 1:4511547/71‑1 (MQ=255)
aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2815675/71‑1 (MQ=255)
aTGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:1925089/71‑1 (MQ=255)
gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacat < 1:453884/47‑1 (MQ=255)
gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAg < 1:1952635/62‑1 (MQ=255)
gACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAgg < 1:2292861/71‑1 (MQ=255)
aCAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatt < 1:489821/47‑1 (MQ=255)
gCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc < 1:1384268/52‑1 (MQ=255)
gCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAggggg > 1:1240268/1‑70 (MQ=255)
cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtgcattaca < 1:1300094/46‑1 (MQ=255)
cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:1596114/1‑44 (MQ=255)
cAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCtacatta > 1:2922384/1‑44 (MQ=255)
cAAACAAGGGTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGc < 1:784762/62‑1 (MQ=255)
aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATc > 1:254766/1‑46 (MQ=255)
aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAgg > 1:3937654/1‑64 (MQ=255)
aaCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAg > 1:169874/1‑71 (MQ=255)
aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4400593/59‑1 (MQ=255)
aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:1017532/60‑1 (MQ=255)
aCAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGt < 1:1561389/71‑1 (MQ=255)
cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:3643905/1‑57 (MQ=255)
cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:660547/1‑57 (MQ=255)
cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:665964/1‑57 (MQ=255)
cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt < 1:4065197/71‑1 (MQ=255)
cAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt > 1:1455605/1‑71 (MQ=255)
aaGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg > 1:1214813/1‑58 (MQ=255)
aGGTTGCTCGATATCTCTACATTTCTGTTTCtgcattaca > 1:574003/1‑40 (MQ=255)
aGGTTGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTc > 1:1003719/1‑71 (MQ=255)
ttGCTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCaa < 1:1572608/71‑1 (MQ=255)
ttGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGcc > 1:3443123/1‑42 (MQ=255)
tGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCa > 1:4159031/1‑69 (MQ=255)
gCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCaaa < 1:1834328/70‑1 (MQ=255)
cTCGATATCTCTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAg < 1:2022369/70‑1 (MQ=255)
cGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGtt > 1:1920804/1‑69 (MQ=255)
tatCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAg < 1:1559010/48‑1 (MQ=255)
tatCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACt > 1:1872640/1‑71 (MQ=255)
atCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGc > 1:4120693/1‑40 (MQ=255)
tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGc < 1:4257072/39‑1 (MQ=255)
tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGc < 1:1930868/39‑1 (MQ=255)
tctcTACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTg < 1:4503973/70‑1 (MQ=255)
tctcTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2373913/44‑1 (MQ=255)
tctcTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTg < 1:956742/70‑1 (MQ=255)
ctctACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGa > 1:971645/1‑71 (MQ=255)
tctACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGa > 1:2864823/1‑43 (MQ=255)
tctACATTTCTGTTTCTACATTACATCGCCGTCTAGCa < 1:808391/38‑1 (MQ=255)
tctACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGAc > 1:3700129/1‑71 (MQ=255)
tACATTTCTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAg < 1:4077149/42‑1 (MQ=255)
tACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAg > 1:3260086/1‑38 (MQ=255)
tACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCg < 1:2377175/40‑1 (MQ=255)
aCATTTCTGTTTCTACATTACATCGCCGTCTAGAAAGCGAGGGGGTAAGTTTCCAAAGta > 1:1256083/1‑60 (MQ=255)
cATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAg > 1:1714413/1‑40 (MQ=255)
cATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGa < 1:4370908/69‑1 (MQ=255)
tttctgtttctgCATTACATCGCCGTCTAGCAAGCg < 1:1328201/36‑1 (MQ=255)
tttctgtttctgCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGtt > 1:3478737/1‑48 (MQ=255)
tttCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:3861568/35‑1 (MQ=255)
tttCTGTTTCTACATTACATCGCCGTCTAGCAAGc < 1:4548070/35‑1 (MQ=255)
tttCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGAtgt > 1:1466129/1‑70 (MQ=255)
ttCTGTTTCTGCATTACATCGCCGTCTAGCAAGCg < 1:1413752/35‑1 (MQ=255)
ttCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGta < 1:3054718/56‑1 (MQ=255)
tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGa > 1:183141/1‑35 (MQ=255)
tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGa > 1:3187085/1‑35 (MQ=255)
tCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAg > 1:3209842/1‑71 (MQ=255)
cTGTTTCTGCATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATAc > 1:3600105/1‑57 (MQ=255)
cTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAgg > 1:1985428/1‑71 (MQ=255)
tCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTa < 1:3218828/69‑1 (MQ=255)
acattacatCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAaataa > 1:1852721/1‑71 (MQ=255)
|
CTGCTATCCGAACCCGGCACAAAATGGACAGCAAACAAGGTTGCTCGATATCTCTACATTTCTGTTTCTACATTACATCGCCGTCTAGCAAGCGAGGGGGTAAGTTTCCAAAGTATACTGGACGATGTGAGGTTAAATAA > W3110S.gb/571187‑571326
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
