Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,128,408 |
T→C |
25.7% |
D155G (GAC→GGC) |
yghO ← |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,128,408 | 0 | T | C | 25.7%
| 122.1
/ 49.5
| 74 | D155G (GAC→GGC) | yghO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/34); new base C (5/14); total (26/48) |
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01 |
TAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGC > W3110S.gb/3128339‑3128478
|
tAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTc < 1:2546889/71‑1 (MQ=255)
aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaa < 1:896080/71‑1 (MQ=255)
aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaa < 1:1644942/71‑1 (MQ=255)
aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCa > 1:447401/1‑70 (MQ=255)
ccAGTTATTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaaaa > 1:3140074/1‑71 (MQ=255)
gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:2716371/1‑70 (MQ=255)
gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:3042197/1‑70 (MQ=255)
gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:1511797/1‑70 (MQ=255)
tCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:454333/1‑71 (MQ=255)
cAATATGCGCGGCATTCCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2396814/70‑1 (MQ=255)
cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1410784/1‑70 (MQ=255)
cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:3026025/1‑70 (MQ=255)
cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1010685/1‑70 (MQ=255)
cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1170702/1‑70 (MQ=255)
atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaaa < 1:3021170/59‑1 (MQ=255)
atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:1547825/71‑1 (MQ=255)
atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAg < 1:3134353/71‑1 (MQ=255)
tGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa > 1:2877669/1‑70 (MQ=255)
gcgGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATcc < 1:268364/71‑1 (MQ=255)
ggCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAACCCTTCAATCAGTAATCCGc < 1:1847164/71‑1 (MQ=255)
ggCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGc < 1:1423599/71‑1 (MQ=255)
tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc < 1:3416303/53‑1 (MQ=255)
tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2267246/56‑1 (MQ=255)
tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:1253135/56‑1 (MQ=255)
ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:4606467/71‑1 (MQ=255)
ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:3801991/1‑71 (MQ=255)
ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:1260114/1‑71 (MQ=255)
ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:3928427/1‑71 (MQ=255)
aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:2590085/1‑52 (MQ=255)
ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:486226/53‑1 (MQ=255)
ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:605958/53‑1 (MQ=255)
ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:256894/53‑1 (MQ=255)
ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:316068/1‑71 (MQ=255)
gctttGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:2223768/51‑1 (MQ=255)
gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattg < 1:4591705/71‑1 (MQ=255)
gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:4442749/1‑70 (MQ=255)
gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:3857442/66‑1 (MQ=255)
gCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGata < 1:3258283/70‑1 (MQ=255)
ccGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATAtt < 1:223352/71‑1 (MQ=255)
gtgtGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:536490/65‑1 (MQ=255)
tgtgGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2117457/41‑1 (MQ=255)
tgGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga > 1:619582/1‑62 (MQ=255)
gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:2553946/1‑56 (MQ=255)
gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCt > 1:827862/1‑70 (MQ=255)
gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:422615/71‑1 (MQ=255)
gCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:3286354/71‑1 (MQ=255)
gCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:2172188/71‑1 (MQ=255)
catcatCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:2528472/71‑1 (MQ=255)
catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:1992104/71‑1 (MQ=255)
catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:3239354/71‑1 (MQ=255)
catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:2279645/71‑1 (MQ=255)
catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:947044/71‑1 (MQ=255)
atcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:1652881/71‑1 (MQ=255)
tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTaa < 1:909812/40‑1 (MQ=255)
tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTa < 1:2494456/39‑1 (MQ=255)
tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCt < 1:1390788/46‑1 (MQ=255)
tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:1200295/70‑1 (MQ=255)
tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:4439608/70‑1 (MQ=255)
catcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:2388035/56‑1 (MQ=255)
atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgat < 1:4067205/52‑1 (MQ=255)
atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgat < 1:4106111/52‑1 (MQ=255)
atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGata > 1:2815054/1‑57 (MQ=255)
atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:4006463/1‑70 (MQ=255)
atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa < 1:3163705/37‑1 (MQ=255)
atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa < 1:2053007/37‑1 (MQ=255)
atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga > 1:1333370/1‑55 (MQ=255)
atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATAtt > 1:4082219/1‑59 (MQ=255)
atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg < 1:4407743/70‑1 (MQ=255)
atcGCACAGGGGGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAgg < 1:3357568/71‑1 (MQ=255)
tcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTaa < 1:481859/37‑1 (MQ=255)
tcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:736359/66‑1 (MQ=255)
cGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAAt > 1:4447979/1‑37 (MQ=255)
aGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTg < 1:995930/71‑1 (MQ=255)
ggtggtGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGAt > 1:4334674/1‑71 (MQ=255)
gtgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:3402387/55‑1 (MQ=255)
tgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAgg < 1:3450088/57‑1 (MQ=255)
tgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTAc < 1:3082554/71‑1 (MQ=255)
tgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg < 1:2834470/54‑1 (MQ=255)
tCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:837537/37‑1 (MQ=255)
tCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:209960/1‑35 (MQ=255)
tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACtt > 1:739633/1‑69 (MQ=255)
tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTg > 1:333334/1‑70 (MQ=255)
tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGc < 1:1843229/71‑1 (MQ=255)
|
TAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGC > W3110S.gb/3128339‑3128478
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
